Transcript: Human XR_001746421.1

PREDICTED: Homo sapiens family with sequence similarity 189 member A2 (FAM189A2), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM189A2 (9413)
Length:
2904
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001746421.1
NBCI Gene record:
FAM189A2 (9413)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001746421.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000275872 ACCGCGGTCTTTGATAGATTA pLKO_005 2803 3UTR 100% 13.200 18.480 N FAM189A2 n/a
2 TRCN0000130658 CCTAACATACCTGCCGAAGAA pLKO.1 819 3UTR 100% 4.950 6.930 N FAM189A2 n/a
3 TRCN0000127971 GCCTTGAAACTCTTTCCGGTT pLKO.1 303 3UTR 100% 2.160 3.024 N FAM189A2 n/a
4 TRCN0000275871 GCCTTGAAACTCTTTCCGGTT pLKO_005 303 3UTR 100% 2.160 3.024 N FAM189A2 n/a
5 TRCN0000128646 CCTCTTAAATCTAGCTGGATT pLKO.1 152 3UTR 100% 4.950 3.960 N FAM189A2 n/a
6 TRCN0000127807 GCTCGAATCAAAGGTGTGGAA pLKO.1 642 3UTR 100% 2.640 2.112 N FAM189A2 n/a
7 TRCN0000275873 CTCCGCTACCTCCAGATATTC pLKO_005 423 3UTR 100% 13.200 9.240 N FAM189A2 n/a
8 TRCN0000275870 GAGATCCTGCATCGATGAATC pLKO_005 452 3UTR 100% 10.800 7.560 N FAM189A2 n/a
9 TRCN0000130756 GAAGTGTTCTGTCCTCTGGAT pLKO.1 660 3UTR 100% 2.640 1.848 N FAM189A2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001746421.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02158 pDONR223 100% 42.2% None (many diffs) n/a
2 ccsbBroad304_02158 pLX_304 0% 42.2% V5 (many diffs) n/a
3 TRCN0000478928 TACTGTGACCAATTTTTATACCCA pLX_317 31.9% 42.2% V5 (many diffs) n/a
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