Construct: ORF TRCN0000478934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000296.2_s317c1
- Derived from:
- ccsbBroadEn_01656
- DNA Barcode:
- ACCACCTGGCATTACCAGCTGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCTE3 (6991)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6991 | TCTE3 | t-complex-associated-testis... | NM_174910.3 | 100% | 100% | |
| 2 | human | 6991 | TCTE3 | t-complex-associated-testis... | XM_011536093.3 | 100% | 100% | |
| 3 | human | 6991 | TCTE3 | t-complex-associated-testis... | XM_006715554.3 | 69.6% | 69.6% | 209_210ins180 |
| 4 | mouse | 100041639 | Gm3448 | predicted gene 3448 | NM_001123367.1 | 84.2% | 79.2% | (many diffs) |
| 5 | mouse | 21647 | Tcte3 | t-complex-associated testis... | NM_011560.3 | 84.2% | 79.2% | (many diffs) |
| 6 | mouse | 100041586 | Gm3417 | predicted gene 3417 | NM_001123368.1 | 84% | 78.7% | (many diffs) |
| 7 | mouse | 21647 | Tcte3 | t-complex-associated testis... | NM_198104.2 | 61.2% | 56% | (many diffs) |
| 8 | mouse | 100041586 | Gm3417 | predicted gene 3417 | XM_006523369.1 | 61.2% | 56% | (many diffs) |
| 9 | mouse | 100041639 | Gm3448 | predicted gene 3448 | XM_006523376.1 | 61.2% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 663
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tcggcaccat ggagaagcga ggccgaggcg tgaagtcgag ccccatccag accccgaacc 121 agacccctca gcaggctccg gtgacgccta ggaaagaaag gaggCCTAGC ATGTTCGAGA 181 AGGAGGCATA TACACAGATT TTAAGAGAAA GACTGAGAGA GTCAATTCAC AATGTTCAGT 241 ATGTGGAGCC TCCTTTTGAT GACTCAATTG CTGATATAGG TAAAGAATGG AAGAGTGCCC 301 TGGCAAAATT AAAGTTTGCT AATTCATATA GAATGGAGCC ATTGAAGAAA TTCCAAGCTC 361 ATTCAGTAGA AACTAAAGTC CAGCAGATAC TAACAGAAAG TCTTAAAGAT GTCAAATATG 421 ATGATAAAGT ATTCTCTCAC TTGTCACTTG AATTGGCAGA CCGCATACTG TTAGCAGTCA 481 AAGAATTTGG GTACCACCGT TATAAGTTCA TTATAAAAGT ATTATTTATT CAAAAGACTG 541 GCCAAGCAAT AAATATTGCC AGCAGATGGA TCTGGGACAT TGCATGGGAC AGCTGGGTCG 601 CAGCTAAACA CGAAGCAGAA TCCTACGTGG CACTGGTCTT GGTGTTTGCC CTTTATTATG 661 AATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAAC CACCTGGCAT TACCAGCTGA ATACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt