Construct: ORF TRCN0000478985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013777.1_s317c1
- Derived from:
- ccsbBroadEn_12939
- DNA Barcode:
- TGCGCGCTCAGAACACTTCTACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF15 (90379)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90379 | DCAF15 | DDB1 and CUL4 associated fa... | NM_138353.3 | 72.6% | 72.6% | 1_492del |
| 2 | mouse | 212123 | Dcaf15 | DDB1 and CUL4 associated fa... | XM_011248336.1 | 77.7% | 81% | (many diffs) |
| 3 | mouse | 212123 | Dcaf15 | DDB1 and CUL4 associated fa... | XM_006530808.1 | 61.6% | 64% | (many diffs) |
| 4 | mouse | 212123 | Dcaf15 | DDB1 and CUL4 associated fa... | NM_172502.3 | 58% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct catgaacatg atgatgatga gtgacgagaa ccaccgtgac atctacgtca 121 gcaccgtggc cgtgccaccg ccaggccgct gtgctgcttg ccaggatgcc agccgagccc 181 acccaggaga cccgaatgca cagtgcctac ggcatggctt catgctgcac accaagtacc 241 aggtggtcta ccccttcccc accttccagc ccgccttcca gctcaagaag gaccaggtgg 301 tgctgctcaa caccagctac tccctggtgg cctgcgccgt ctccgtccac tcggcaggtg 361 acaggagttt ctgccaaatc ctgtatgacc acagcacctg ccccctggcg cctgccagcc 421 cccctgagcc ccagagccca gagctgcccc ctgccctccc cagcttctgc cctgaggcgg 481 ccccagcccg ttcttctggg tctcctgagc cctcgcccgc cattgccaaa gccaaggagt 541 ttgtggctga catcttccgc cgggccaaag aggccaaggg cggggtccct gaggaagccc 601 ggcctgccct gtgcccagga ccctctggca gccgctgccg tgcgcactct gagcccctag 661 ccctgtgtgg agagacggca ccccgggaca gcccccctgc ctcggaggca cctgcctccg 721 agcctggcta tgtcaactac accaagctgt actatgtgct ggagtccgga gaggggacgg 781 agccggagga tgagttggag gacgacaaga tctccctgcc cttcgtggtg actgatcttc 841 gtggccgcaa cctgcggccc atgcgggagc ggactgctgt ccagggccag tacctgacag 901 tggagcagct cacactagac ttcgaatatg ttatcaatga ggtcatccgc cacgacgcta 961 cctggggcca tcagttctgt tctttcagcg actatgacat cgtcattctg gaggtctgcc 1021 cagaaaccaa ccaggtcctc atcaacattg gcctgctgcT CCTGGCCTTC CCGTCCCCCA 1081 CTGAGGAGGG CCAGCTCCGA CCAAAGACCT ATCACACCAG CCTCAAGGTG GCATGGGACC 1141 TCAACACAGG GATCTTCGAG ACAGTCAGTG TAGGCGACCT GACTGAGGTC AAAGGGCAGA 1201 CCAGCGGCAG TGTCTGGAGC TCCTACCGCA AGAGCTGCGT GGACATGGTC ATGAAGTGGC 1261 TGGTGCCGGA GAGCAGCGGC CGCTACGTCA ACAGGATGAC CAATGAGGCG CTGCACAAAG 1321 GGTGCTCCCT GAAGGTTCTG GCGGACAGCG AGCGATATAC GTGGATCGTG CTGTGCCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAT GCGCGCTCAG AACACTTCTA CCCACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att