Construct: ORF TRCN0000478998
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011043.1_s317c1
- Derived from:
- ccsbBroadEn_12920
- DNA Barcode:
- AGTGAAAAATCGTGCTCGTGCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM4 (89122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478998
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89122 | TRIM4 | tripartite motif containing 4 | NM_033091.3 | 60.9% | 59.1% | (many diffs) |
| 2 | human | 89122 | TRIM4 | tripartite motif containing 4 | NM_033017.4 | 57.7% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 948
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agctgaggac atccaggagg agttgacctg ccccatctgc ctggactatt 121 tccaggaccc ggtgtccatc gagtgcggcc acaacttctg ccgcggctgc ctgcaccgca 181 actgggcgcc gggcggcggc ccgttcccct gccccgaatg tcggcaccca tcggcgcccg 241 ccgcgctgcg acccaactgg gccctggcca ggctgactga gaagacgcag cgccggcgcc 301 tgggccccgt gcccccgggc ctgtgcggcc gccactggga gccgctgcgg ctcttctgcg 361 aggacgacca gcggccagtg tgcctggtgt gcagggagtc ccaggagcac cagactcacg 421 ccatggcacc catcgacgag gccttCGAGA GCTACCGGGA GAAACTTCTT AAGTCTCAGC 481 GTAATCTCGT GGCCAAGATG AAGAAAGTCA TGCATTTACA GGATGTAGAA GTGAAGAACG 541 CCACACAGTG GAAGGATAAG ATAAAGAGTC AGCGAATGAG AATCAGCACG GAGTTTTCAA 601 AGCTGCACAA CTTCCTGGTT GAAGAAGAGG ACCTGTTTCT TCAGAGATTG AACAAAGAAG 661 AAGAAGAGAC GAAGAAGAAG CTGAATGAGA ACACGTTAAA ACTCAATCAA ACTATCGCTT 721 CATTGAAGAA GCTCATCTTA GAGGTGGGGG AGAAGAGCCA GGCTCCCACC CTGGAGCTGC 781 TTCAGAATCC AAAAGAAGTG TTGACCAGGA GTGAGATCCA GGATGTGAAC TATTCTCTTG 841 AAGCTGTAAA GGTGAAGACA GTGTGCCAGA TACCATTGAT GAAGGAAATG CTAAAGCGAT 901 TCCAAGCAAC TAAAATAAAA ACACAAAAAC AAAACAGGCA CAATATGTGC CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGAAGTGAAA AATCGTGCTC GTGCGTGACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt