Construct: ORF TRCN0000479000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006311.2_s317c1
- Derived from:
- ccsbBroadEn_07873
- DNA Barcode:
- TATTGACGCACACCCCGATCTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UFL1 (23376)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23376 | UFL1 | UFM1 specific ligase 1 | NM_015323.5 | 99.9% | 100% | 168T>C |
| 2 | mouse | 67490 | Ufl1 | UFM1 specific ligase 1 | NM_026194.4 | 86.9% | 90.5% | (many diffs) |
| 3 | mouse | 67490 | Ufl1 | UFM1 specific ligase 1 | XM_006538203.3 | 86.9% | 90.5% | (many diffs) |
| 4 | mouse | 67490 | Ufl1 | UFM1 specific ligase 1 | XM_006538204.3 | 49.5% | 50.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2448
- ORF length:
- 2382
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgcctgg gaagagatta ggcggttggc ggccgacttc cagcgggcgc 121 agttcgccga ggccacgcag aggttgtccg agcggaactg cattgagatt gttaataaat 181 tgattgctca gaaacagcta gaagtagttc atacactcga tggaaaggaa tacattactc 241 cagcccaaat tagtaaagaa atgagagatg agctacatgt ccgaggtggt cgagtaaaca 301 ttgttgatct acaacaggta attaatgtgg acctgattca tattgaaaat agaattggtg 361 acattattaa atcagaaaag catgttcagt tagtgttggg acaactgata gatgagaatt 421 atttggatcg gttggcagaa gaggtcaatg ataaattgca agaaagtggt caggtcacca 481 tatcagaact gtgtaaaact tatgatcttc ctgggaactt tctgacacag gcactaactc 541 agcgacttgg tagaattatc agtggacata ttgatcttga taatagagga gtaattttta 601 cggaagcttt tgtagctcga cataaagcac gtatccgtgg actattcagt gctattaccc 661 ggcctacagc tgtgaattct ttgatttcaa aatatggatt tcaggagcag cttctttact 721 ctgtgcttga ggaacttgtt aatagcggac gcttacgagg cactgtggtt ggtgggagac 781 aggataaagc tgtgtttgtc cctgacatct actccaggac acagagtact tgggtggatt 841 cctttttcag gcagaatggc tatctagaat ttgatgcttt gtccagactt ggaatcccag 901 atgctgtaag ctacataaag aaaagatata agactacaca actcttgttt ttgaaagcag 961 cttgtgttgg tcaaggactt gtggatcaag tggaagcatc agtagaagaa gccatcagct 1021 ctggaacatg ggttgatatt gcacctctgc tacccacttc tttatcagtt gaagatgctg 1081 ccatattgct tcagcaggtg atgagggcat tcagcaaaca ggcctcaact gtagtcttta 1141 gcgacactgt tgtagtcagt gaaaaattta taaatgactg tacagaactg ttccgtgagc 1201 tgatgcacca gaaagctgaa aaggaaatga aaaataatcc tgtgcattta atcactgaag 1261 aagatctgaa acaaatctcc actttagaaa gcgttagtac aagtaaaaag gataaaaaag 1321 atgagcgaag aaggaaagca acagagggca gtggaagcat gagaggagga ggtgggggca 1381 atgccagaga gtacaaaatt aaaaaagtca agaagaaagg aagaaaagat gatgatagtg 1441 atgatgaatc tcaatcatcc cacactggaa agaagaagcc agagatcagt tttatgttcc 1501 aggatgagat tgaagatttt ttaagaaaac acatacaaga tgcccctgag gagtttattt 1561 cggaacttgc tgagtactta ataaaacctc ttaataaaac ttatctcgag gtggtacgtt 1621 cagtattcat gtcttcaaca acttctgctt ctgggacggg cagaaaacgc acaatcaagg 1681 acttgcaaga agaagtttca aacctgtaca ataacattag gttatttgaa aaagggatga 1741 agttttttgc agatgacaca caggctgctc ttaccaaaca cttgctgaag tcagtgtgta 1801 ctgatatcac taacctcatt ttcaacttct tagcttcgga tttaatgatg gcagtagacg 1861 atcctgcagc cattacaagt gaaataagaa agaaaatttt aagtaaatta tcagaagaaa 1921 ccaaagtagc tcttacaaaa ctccataact ctctgaatga aaagagcata gaagacttta 1981 tttcttgtct ggattcTGCA GCAGAAGCTT GTGATATTAT GGTGAAAAGG GGAGACAAAA 2041 AAAGGGAAAG ACAGATACTG TTCCAACATC GACAAGCACT GGCTGAACAG CTAAAGGTCA 2101 CAGAAGACCC TGCTCTTATT CTGCACCTCA CATCAGTCCT GTTGTTTCAG TTTTCAACCC 2161 ACAGCATGCT CCATGCACCT GGAAGATGTG TCCCACAGAT CATTGCTTTT CTTAATAGTA 2221 AAATTCCAGA GGATCAGCAT GCTCTTTTGG TAAAGTATCA AGGTTTGGTT GTAAAGCAGC 2281 TAGTCAGTCA AAGTAAGAAG ACTGGGCAGG GAGATTATCC CTTGAATAAT GAATTAGACA 2341 AAGAACAAGA AGATGTTGCC AGTACTACTC GTAAAGAGCT TCAAGAACTT TCTTCATCCA 2401 TTAAAGACCT TGTTCTCAAA TCTAGGAAAT CATCTGTGAC GGAAGAGTAC CCAACTTTCT 2461 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2521 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2581 GTGGAAAGGA CGATATTGAC GCACACCCCG ATCTATTACG CGTTAAGTCg acaatcaacc 2641 tctggattac aaaatttgtg aaagatt