Construct: ORF TRCN0000479010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010510.1_s317c1
- Derived from:
- ccsbBroadEn_00821
- DNA Barcode:
- GCCTTAGGGCGGCCTGGCCATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA1 (3439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479010
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 100% | 100% | |
| 2 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 99.2% | 98.9% | 1_3delATG;32C>T |
| 3 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 90.8% | 84.6% | (many diffs) |
| 4 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 90.2% | 82% | (many diffs) |
| 5 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 90.1% | 84.1% | (many diffs) |
| 6 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 89.5% | 82% | (many diffs) |
| 7 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 89.4% | 80.9% | (many diffs) |
| 8 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 88.3% | 79.8% | (many diffs) |
| 9 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 88.3% | 78.8% | (many diffs) |
| 10 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 87.6% | 78.8% | (many diffs) |
| 11 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 87.6% | 77.2% | (many diffs) |
| 12 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 87.1% | 76.7% | (many diffs) |
| 13 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 86.9% | 76.7% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcgccc tttgctttac tgatggtcct ggtggtgctc agctgcaagt 121 caagctgctc tctgggctgt gatctccctg agacccacag cctggataac aggaggacct 181 tgatgctcct ggcacaaatg agcagaatct ctccttccTC CTGTCTGATG GACAGACATG 241 ACTTTGGATT TCCCCAGGAG GAGTTTGATG GCAACCAGTT CCAGAAGGCT CCAGCCATCT 301 CTGTCCTCCA TGAGCTGATC CAGCAGATCT TCAACCTCTT TACCACAAAA GATTCATCTG 361 CTGCTTGGGA TGAGGACCTC CTAGACAAAT TCTGCACCGA ACTCTACCAG CAGCTGAATG 421 ACTTGGAAGC CTGTGTGATG CAGGAGGAGA GGGTGGGAGA AACTCCCCTG ATGAATGCGG 481 ACTCCATCTT GGCTGTGAAG AAATACTTCC GAAGAATCAC TCTCTATCTG ACAGAGAAGA 541 AATACAGCCC TTGTGCCTGG GAGGTTGTCA GAGCAGAAAT CATGAGATCC CTCTCTTTAT 601 CAACAAACTT GCAAGAAAGA TTAAGGAGGA AGGAATTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AGCCTTAGGG CGGCCTGGCC ATAATACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt