Construct: ORF TRCN0000479024
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008069.1_s317c1
- Derived from:
- ccsbBroadEn_12937
- DNA Barcode:
- GAAAGCACCAAAATCCTTTAACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF616 (90317)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479024
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400950 | C2orf91 | chromosome 2 open reading f... | NR_161189.1 | 3.2% | (many diffs) | |
| 2 | human | 90317 | ZNF616 | zinc finger protein 616 | NR_135823.1 | 3.1% | 1_3721del;3863_4484del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 222
- ORF end:
- 363
- ORF length:
- 141
- Sequence:
-
1 GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC 61 AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT 121 CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG 181 CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGGGATAC TATGCAGCCA 241 TAGAAAGGAA TGAGATCATG TCCATTGCAG CGATGTGGAT GGAGCTGGAA GCCATTATCC 301 TCAGGAAACT AACACAGGAA CAGAAAATCA AACAGCACAT GTTCTCACTT AAAAGTGGAA 361 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGAGA AAGCACCAAA ATCCTTTAAC ATACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt