Construct: ORF TRCN0000479025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007207.1_s317c1
- Derived from:
- ccsbBroadEn_10441
- DNA Barcode:
- TCCTCACTCTGAATTCCGACCTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PEPD (5184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5184 | PEPD | peptidase D | NM_000285.4 | 99.7% | 16G>T;1303C>T;1477A>C | |
| 2 | human | 5184 | PEPD | peptidase D | NM_001166056.2 | 91.4% | (many diffs) | |
| 3 | human | 5184 | PEPD | peptidase D | NM_001166057.2 | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 81
- ORF length:
- 15
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggccacc tgaccctcgt tttggctggg gaatgaaacc ctgaaggtgc 121 cgctggcgct ctttgccttg aaccggcagc gcctgtgtga gcggctgcgg aagaaccctg 181 ctgtgcaggc cggctccatc gtggtcctgc agggcgggga ggagactcag cgctactgca 241 ccgacaccgg ggtcctcttc cgccaggagt ccttctttca ctgggcgttc ggtgtcactg 301 agccaggctg ctatggtgtc atcgatgttg acactgggaa gtcgaccctg tttgtgccca 361 ggcttcctgc cagccatgcc acctggatgg gaaagatcca ttccaaggag cacttcaagg 421 agaagtatgc cgtggacgac gtccagtacg tagatgagat tgccagcgtc ctgacgtcac 481 agaagccctc tgtcctcctc actttgcgtg gcgtcaacac ggacagcggc agtgtctgca 541 gggaggcctc ctttgacggc atcagcaagt tcgaagtcaa caataccatt cttcacccag 601 agatcgttga gtgccgagtg tttaagacgg atatggagct ggaggttctg cgctatacca 661 ataaaatctc cagcgaggcc caccgtgagg taatgaaggc tgtaaaagtg ggaatgaaag 721 aatatgagtt ggaaagcctc ttcgagcact actgctactc ccggggcggc atgcgccaca 781 gctcctacac ctgcatctgc ggcagtggtg agaactcagc cgtgctacac tacggacacg 841 ccggagctcc caacgaccga acgatccaga atggggatat gtgcctgttc gacatgggcg 901 gtgagtatta ctgcttcgct tccgacatca cctgctcctt tcccgccaac ggcaagttca 961 ctgcagacca gaaggccgtc tatgaggcag tgctgcggag ctcccgtgcc gtcatgggtg 1021 ccatgaagcc aggtgtctgg tggcctgaca tgcaccgcct ggctgaccgc atccacctgg 1081 aggagctggc ccacatgggc atcctgagcg gcagcgtgga cgccatggtc caggctcacc 1141 tgggggccgt gtttatgcct cacgggcttG GCCACTTCCT GGGCATTGAC GTGCACGACG 1201 TGGGAGGCTA CCCAGAGGGC GTGGAGCGCA TCGACGAGCC CGGCCTGCGG AGCCTGCGCA 1261 CTGCACGGCA CCTGCAGCCA GGCATGGTGC TCACCGTGGA GCCGGGCATC TACTTCATCG 1321 ACCACCTCCT GGATGAGGCC CTGGCGGACC CGGCCCGCGC CTCCTTCTTT AACCGCGAGG 1381 TCCTGCAGCG CTTTCGCGGT TTTGGCGGGG TCCGCATCGA GGAGGACGTC GTGGTGACTG 1441 ACAGCGGCAT AGAGCTGCTG ACCTGCGTGC CCCGCACTGT GGAAGAGATT GAAGCATGCA 1501 TGGCAGGCTG TGACAAGGCC TTTACCCCCT TCTCTGGCCC CCAGTACCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TCCTCACTCT GAATTCCGAC CTCGACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt