Construct: ORF TRCN0000479043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012785.1_s317c1
- Derived from:
- ccsbBroadEn_09268
- DNA Barcode:
- CTTTTACCTGAGTGCGAGCTCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSKH2 (85481)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85481 | PSKH2 | protein serine kinase H2 | NM_033126.3 | 100% | 100% | |
| 2 | human | 85481 | PSKH2 | protein serine kinase H2 | XM_017013929.1 | 76.3% | 68.6% | 1_286del;470_540del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1224
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtgcggc gccagcagga aggtggtccc ggggccacca gcgctggctt 121 gggccaagca cgaaggtcaa aaccaagccg gcgtcggagg cgcggggcct gggcccgagg 181 cggcggccca ggcggcgcag aggatacagg tggctcgctt ccgagccaag ttcgaccccc 241 gggtccttgc cagatatgac atcaaagctc ttattgggac aggcagtttc agcagggttg 301 tcagggtaga gcagaagacc accaagaaac cttttgcaat aaaagtgatg gaaaccagag 361 agagggaagg tagagaagcg tgcgtgtctg agctgagcgt cctgcggcgg gttagccatc 421 gttacattgt ccagctcatg gagatctttg agactgagga tcaagtttac atggtaatgg 481 agctggctac cggaggggag ctctttgatc gactcattgc tcagggatcc tttacagagc 541 gggatgccgt caggatcctc cagatggttg ctgatgggat taggtatttg catgcgctgc 601 agataactca taggaatcta aagcctgaaa acctcttata ctatcatcca ggtgaagagt 661 cgaaaatttt aattacagat tttggtttgg catactccgg gaaaaaaagt ggtgactgga 721 caatgaagac actctgtggg accccagagt acatagctcc tgaggttttg ctaaggaagc 781 cTTATACCAG TGCAGTGGAC ATGTGGGCTC TTGGTGTGAT CACATATGCT TTACTTAGCG 841 GATTCCTGCC TTTTGATGAT GAAAGCCAGA CAAGGCTTTA CAGGAAGATT CTGAAAGGCA 901 AATATAATTA TACAGGAGAG CCTTGGCCAA GCATTTCCCA CTTGGCGAAG GACTTTATAG 961 ACAAACTACT GATTTTGGAG GCTGGTCATC GCATGTCAGC TGGCCAGGCC CTGGACCATC 1021 CCTGGGTGAT CACCATGGCT GCAGGGTCTT CCATGAAGAA TCTCCAGAGG GCCATATCCC 1081 GAAACCTCAT GCAGAGGGCC TCTCCCCACT CTCAGAGTCC TGGATCTGCA CAGTCTTCTA 1141 AGTCACATTA TTCTCACAAA TCCAGGCATA TGTGGAGCAA GAGAAACTTA AGGATAGTAG 1201 AATCGCCACT GTCTGCGCTT TTGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TTTTACCTGA 1381 GTGCGAGCTC CGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att