Transcript: Human NM_033126.3

Homo sapiens protein serine kinase H2 (PSKH2), mRNA.

Source:
NCBI, updated 2019-05-17
Taxon:
Homo sapiens (human)
Gene:
PSKH2 (85481)
Length:
2557
CDS:
47..1204

Additional Resources:

NCBI RefSeq record:
NM_033126.3
NBCI Gene record:
PSKH2 (85481)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147017 TGAGCTGGACAATGTAACGA pXPR_003 TGG 353 30% 2 0.9274 PSKH2 PSKH2 77266
2 BRDN0001146562 TGGACAATGAAGACACTCTG pXPR_003 TGG 665 57% 2 -0.0760 PSKH2 PSKH2 77267
3 BRDN0001147887 CTCAGGGATCCTTTACAGAG pXPR_003 CGG 468 40% 2 -0.2144 PSKH2 PSKH2 77268
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033126.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003215 CGGGTTAGCCATCGTTACATT pLKO.1 386 CDS 100% 5.625 7.875 N PSKH2 n/a
2 TRCN0000003216 TGATGGGATTAGGTATTTGCA pLKO.1 550 CDS 100% 3.000 4.200 N PSKH2 n/a
3 TRCN0000199194 CAGAGGGCCATATCCCGAAAC pLKO.1 1043 CDS 100% 2.000 2.800 N PSKH2 n/a
4 TRCN0000199480 GAGAGCCTTGGCCAAGCATTT pLKO.1 894 CDS 100% 10.800 7.560 N PSKH2 n/a
5 TRCN0000195178 CATAGGAATCTAAAGCCTGAA pLKO.1 587 CDS 100% 4.050 2.835 N PSKH2 n/a
6 TRCN0000003217 GAGGATCAAGTTTACATGGTA pLKO.1 434 CDS 100% 3.000 2.100 N PSKH2 n/a
7 TRCN0000003214 GAGCAGAAGACCACCAAGAAA pLKO.1 287 CDS 100% 5.625 3.375 N PSKH2 n/a
8 TRCN0000003218 ACTGGACAATGAAGACACTCT pLKO.1 693 CDS 100% 2.640 1.584 N PSKH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033126.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09268 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_09268 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000479043 CTTTTACCTGAGTGCGAGCTCCGA pLX_317 38.4% 100% 100% V5 n/a
4 TRCN0000487738 AAGGCGTCTGTAGCGGGATATGTA pLX_317 18.4% 100% 100% V5 (not translated due to prior stop codon) n/a
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