Construct: ORF TRCN0000479070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000435.1_s317c1
- Derived from:
- ccsbBroadEn_05072
- DNA Barcode:
- ATAGCAAAATGTTACTTACTCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRFBP1 (153443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479070
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153443 | SRFBP1 | serum response factor bindi... | NM_152546.3 | 100% | 100% | |
| 2 | human | 153443 | SRFBP1 | serum response factor bindi... | XM_017009111.2 | 86.2% | 85.7% | 1106G>A;1108_1108delAinsTTTCCAC;1113_1113delAins169 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcagccggga actctgaacc tcaataacga ggttgtgaag atgagaaaag 121 aagtgaagag aattcgagtt ttagttatcc gaaaacttgt caggagtgtt ggccgactga 181 agtcaaaaaa gggtactgaa gatgcactgt taaaaaacca aagacgggcg caaagattgc 241 ttgaagaaat ccatgccatg aaggaattga aacctgacat agtaactaaa tctgctcttg 301 gtgatgatat caactttgaa aaaatcttca aaaagccaga ttctactgca actgaaagag 361 caattgccag actagcagta catcctcttc tgaagaaaaa gatagatgtg ctaaaagctg 421 ctgtacaagc ctttaaagaa gcaagacaaa atgttgctga agttgagtca tcaaagaatg 481 cttcagagga caatcattct gagaatactt tgtattcaaa tgataatgga agtaatttac 541 agcgtgaagc aactgtcatc agtgagcaaa aagtcaaaga aaccaaaata ttggcgaaga 601 aaccaataca taattcaaag gaaaaaatag caaagatgga acatggacct aaagcagtga 661 ctattgcaaa ttctccatca aagccttcag aaaaggattc tgtagtttcc cttgagtccc 721 agaagacacc tgctgaccca aaactgaaaa ctctaagtca aaccaaaaaa aacaaaggat 781 ctgatagctc actctctggt aacagtgatg gcggagaaga attttgtgaa gaggagaagg 841 aatattttga tgatagcaca gaagaaaggt tttacaagca gtcttccatg tctgaagata 901 gtgatagcgg tgacgacttc ttcattggga aagtcagacg gacacgaaag aaggaaagta 961 gttgtcatTC TTCAGTTAAG GAACAAAAAC CACTAGAAAA AGTGTTTCTT AAAGAAGATA 1021 CAGGTGAAAC TCATGGGGAT ACAAGAAATG ACAAAATCAA GCCAAGTACA GAAACCAGAA 1081 AGTTAGAATC AGTGTTTTTC CACTCTTTAT CTGGATCTAA AAGCTCTAGA AGAAATTTCA 1141 AAGAACAGGC TCCAAAAACA AGATCCCTAG ATTTTCCACA GAATGAGCCT CAGATCAAGA 1201 ATCAGTTTAA TAAGAAGCTA TCAGGAAGAC TTGAAAATAC AAAACAGCAA TTGCAGCTGC 1261 CTCTTCATCC TTCATGGGAA GCAAGCAGAA GGCGAAAAGA ACAGCAATCT AATATTGCTG 1321 TGTTTCAGGG GAAAAAAATT ACGTTTGATG ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 1501 AGCAAAATGT TACTTACTCA GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt