Construct: ORF TRCN0000479077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010155.1_s317c1
- Derived from:
- ccsbBroadEn_06405
- DNA Barcode:
- GGATTGTTCTTGCACGTTAGCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJA1 (3301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3301 | DNAJA1 | DnaJ heat shock protein fam... | NM_001539.4 | 99.9% | 100% | 90T>C |
| 2 | human | 3301 | DNAJA1 | DnaJ heat shock protein fam... | NM_001314039.1 | 60.4% | 60.4% | 0_1ins471 |
| 3 | mouse | 15502 | Dnaja1 | DnaJ heat shock protein fam... | NM_001164671.2 | 92.1% | 99.4% | (many diffs) |
| 4 | mouse | 15502 | Dnaja1 | DnaJ heat shock protein fam... | NM_001164672.2 | 92.1% | 99.4% | (many diffs) |
| 5 | mouse | 15502 | Dnaja1 | DnaJ heat shock protein fam... | NM_008298.6 | 92.1% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1257
- ORF length:
- 1191
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaaagaaaca acttactacg atgttttggg ggtcaaaccc aatgctactc 121 aggaagaatt gaaaaaggct tataggaaac tggccttgaa gtaccatcct gataagaacc 181 caaatgaagg agagaagttt aaacagattt ctcaagctta cgaagttctc tctgatgcaa 241 agaaaaggga attatatgac aaaggaggag aacaggcaat taaagagggt ggagcaggtg 301 gcggttttgg ctcccccatg gacatctttg atatgttttt tggaggagga ggaaggatgc 361 agagagaaag gagaggtaaa aatgttgtac atcagctctc agtaacccta gaagacttat 421 ataatggtgc aacaagaaaa ctggctctgc aaaagaatgt gatttgtgac aaatgtgaag 481 gtagaggagg taagaaagga gcagtagagt gctgtcccaa ttgccgaggt actggaatgc 541 aaataagaat tcatcagata ggacctggaa tggttcagca aattcagtct gtgtgcatgg 601 agtgccaggg ccatggggag cggatcagtc ctaaagatag atgtaaaagc tgcaacggaa 661 ggaagatagt tcgagagaag aaaattttag aagttcatat tgacaaaggc atgaaagatg 721 gccagaagat aacattccat ggtgaaggag accaagaacc aggactggag ccaggcgata 781 ttatcattgt gttagatcag aaggaccatg ctgtttttac tcgacgagga gaagaccttt 841 tcatgtgtat ggacatacag ctcgttgaag cactgtgtgg cttccagaag ccaatatcta 901 ctcttgacaa ccgaaccatc gtcaTCACCT CTCATCCAGG TCAGATTGTC AAGCATGGAG 961 ATATCAAGTG TGTACTAAAT GAAGGCATGC CAATTTATCG TAGACCATAT GAAAAGGGTC 1021 GCCTAATCAT CGAATTTAAG GTAAACTTTC CTGAGAATGG CTTTCTCTCT CCTGATAAAC 1081 TGTCTTTGCT GGAAAAACTC CTACCCGAGA GGAAGGAAGT GGAAGAGACT GATGAGATGG 1141 ACCAAGTAGA ACTGGTGGAC TTTGATCCAA ATCAGGAAAG ACGGCGCCAC TACAATGGAG 1201 AAGCATATGA GGATGATGAA CATCATCCCA GAGGTGGTGT TCAGTGTCAG ACCTCTTACC 1261 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1321 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1381 ATATATCTTG TGGAAAGGAC GAGGATTGTT CTTGCACGTT AGCGGCACGC GTTAAGTCga 1441 caatcaacct ctggattaca aaatttgtga aagatt