Construct: ORF TRCN0000479090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000334.1_s317c1
- Derived from:
- ccsbBroadEn_10766
- DNA Barcode:
- ACGAAGTGCCACACTGTCATTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP19A1 (1588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479090
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_000103.4 | 35.4% | 34.1% | (many diffs) |
| 2 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347248.1 | 35.4% | 34.1% | (many diffs) |
| 3 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347249.2 | 35.4% | 34.1% | (many diffs) |
| 4 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347250.1 | 35.4% | 34.1% | (many diffs) |
| 5 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347251.1 | 35.4% | 34.1% | (many diffs) |
| 6 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347252.1 | 35.4% | 34.1% | (many diffs) |
| 7 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347253.1 | 35.4% | 34.1% | (many diffs) |
| 8 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347254.1 | 35.4% | 34.1% | (many diffs) |
| 9 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347255.2 | 35.4% | 34.1% | (many diffs) |
| 10 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347256.2 | 35.4% | 34.1% | (many diffs) |
| 11 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_031226.3 | 35.4% | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tttggaaatg ctgaacccga tacattataa catcaccagc atcgtgcctg 121 aagccatgcc tgctgccacc atgccagtcc tgctcctcac tggccttttt ctcttggtgt 181 ggaattatga gggcacatcc tcaataccag gtcctggcta ctgcatggga attggacccc 241 tcatctccca cggcagatTC CTGTGGATGG GGATCGGCAG TGCCTGCAAC TACTACAACC 301 GGGTGTATGG AGAATTCATG CGAGTCTGGA TCTCTGGAGA GGAAACACTC ATTATCAGCA 361 AGTCCTCAAG TATGTTCCAC ATAATGAAGC ACAATCATTA CAGCTCTCGA TTCGGCAGCA 421 AACTTGGGCT GCAGTGCATC GGTATGCATG AGAAAGGCAT CATATTTAAC AACAATCCAG 481 AGCTCTGGAA AACAACTCGA CCCTTCTTTA TGAAAGCTCT GTCAGGCCCC GGCCTTGTTC 541 GTATGGTCAC AGTCTGTGCT GAATCCCTCA AAACACATCT GCTCCTGTTC ACACCTGCTA 601 GTGTGAATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACGAAG TGCCACACTG TCATTTGTAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt