Construct: ORF TRCN0000479098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009067.1_s317c1
- Derived from:
- ccsbBroadEn_10020
- DNA Barcode:
- GGCGTATGGGAGTCACTGGATCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMPRSS11A (339967)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339967 | TMPRSS11A | transmembrane serine protea... | NM_001114387.2 | 99.7% | 99.7% | 18G>A;869A>G;1002T>C |
| 2 | human | 339967 | TMPRSS11A | transmembrane serine protea... | NM_182606.4 | 99% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1323
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgtatcgg acagtaggat ttggcacccg aagcagaaat ctgaagccat 121 ggatgattgc cgttctcatt gtgttgtccc tgacagtggt ggcagtgacc ataggtctcc 181 tggttcactt cctagtattt gaccaaaaaa aggagtacta tcatggctcc tttaaaattt 241 tagatccaca aatcaataac aatttcggac aaagcaacac atatcaactt aaggacttac 301 gagagacgac cgaaaatttg gtggatgaga tatttataga ttcagcctgg aagaaaaatt 361 atatcaagaa ccaagtagtc agactgactc cagaggaaga tggtgtgaaa gtagatgtca 421 ttatggtgtt ccagttcccc tctactgaac aaagggcagt aagagagaag aaaatccaaa 481 gcatcttaaa tcagaagata aggaatttaa gagccttgcc aataaatgcc tcatcagttc 541 aagttaatgc aatgagctca tcaacagggg agttaactgt ccaagcaagt tgtggtaaac 601 gagttgttcc attaaacgtc aacagaatag catctggagt cattgcaccc aaggcggcct 661 ggccttggca agcttccctt cagtatgata acatccatca gtgtggggcc accttgatta 721 gtaacacatg gcttgtcact gcagcacact gcttccagaa gtataaaaat ccacatcaat 781 ggactgttag ttttggaaca aaaatcaacc ctcccttaat gaaaagaaat gtcagaagat 841 ttattatcca tgagaagtac cgctctgcag caagagagta cgacattgct gttgtgcagg 901 tcTCTTCCAG AGTCACCTTT TCGGATGACA TACGCCGGAT TTGTTTGCCA GAAGCCTCTG 961 CATCCTTCCA ACCAAATTTG ACTGTCCACA TCACAGGATT TGGAGCACTT TACTATGGTG 1021 GGGAATCCCA AAATGATCTC CGAGAAGCCA GAGTGAAAAT CATAAGTGAC GATGTCTGCA 1081 AGCAACCACA GGTGTATGGC AATGATATAA AACCTGGAAT GTTCTGTGCC GGATATATGG 1141 AAGGAATTTA TGATGCCTGC AGGGGTGATT CTGGGGGACC TTTAGTCACA AGGGATCTGA 1201 AAGATACGTG GTATCTCATT GGAATTGTAA GCTGGGGAGA TAACTGTGGT CAAAAGGACA 1261 AGCCTGGAGT CTACACACAA GTGACTTATT ACCGAAACTG GATTGCTTCA AAAACAGGCA 1321 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGAGG CGTATGGGAG TCACTGGATC AAACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt