Construct: ORF TRCN0000479099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008565.1_s317c1
- Derived from:
- ccsbBroadEn_02862
- DNA Barcode:
- ATCTGCCGAGGGCGAGATCCTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOCT (25819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479099
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25819 | NOCT | nocturnin | NM_012118.4 | 100% | 100% | |
| 2 | mouse | 12457 | Noct | nocturnin | NM_009834.2 | 84.6% | 90.2% | (many diffs) |
| 3 | mouse | 12457 | Noct | nocturnin | XM_006500956.1 | 73.9% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1362
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttcatagt ccgcggcggc tctgctcggc cctgctgcag agggacgcgc 121 ccggcctgcg ccgcctgccc gccccagggc tgcgccgccc gttgtccccg ccggctgctg 181 ttcccaggcc cgcatccccc cggctgctgg cggcggcctc ggcggcctcg ggcgccgcga 241 ggtcgtgttc ccgaacagtg tgttccatgg gaaccggtac aagcagactc tatagtgctc 301 tcgccaagac actgaacagc agcgctgcct cccagcaccc agagtatttg gtgtcacctg 361 acccagagca tctggagccc attgatccta aagagcttct tgaggaatgc agggccgtcc 421 tgcacacccg acctccccgg ttccagaggg attttgtgga tctgaggaca gattgcccta 481 gtacccaccc acctatcagg gttatgcaat ggaacatcct cgcccaagct cttggagaag 541 gcaaagacaa ctttgtacag tgccctgttg aagcactcaa atgggaagaa aggaaatgtc 601 tcatcctgga agaaatcctg gcctaccagc ctgatatatt gtgcctccaa gaggtggacc 661 actattttga caccttccag ccactcctca gtagactagg ctatcaaggc acgtttttcc 721 ccaaaccctg gtcaccttgt ctagatgtag aacacaacaa tggaccagat ggttgtgcct 781 tattttttct tcaaaaccga ttcaagctag tcaacagtgc caatattagg ctgacagcca 841 tgacattgaa aaccaaccag gtggcCATTG CACAGACCCT GGAGTGCAAG GAGTCAGGCC 901 GACAGTTCTG CATCGCTGTT ACCCATCTAA AAGCACGCAC TGGCTGGGAG CGGTTTCGAT 961 CAGCTCAAGG CTGTGACCTC CTTCAGAACC TGCAAAACAT CACCCAAGGA GCCAAGATTC 1021 CCCTTATTGT GTGTGGGGAC TTCAATGCAG AGCCAACAGA AGAGGTCTAC AAACACTTTG 1081 CTTCCTCCAG CCTCAACCTG AACAGCGCCT ACAAGCTGCT GAGTGCTGAT GGGCAGTCAG 1141 AACCCCCATA CACTACCTGG AAGATCCGGA CCTCAGGGGA GTGCAGGCAC ACCCTGGATT 1201 ACATCTGGTA TTCTAAACAT GCTCTAAATG TAAGGTCAGC TCTCGATCTG CTCACTGAAG 1261 AACAGATTGG ACCCAACAGG TTACCTTCCT TCAATTATCC TTCAGACCAC CTGTCTCTAG 1321 TGTGTGACTT CAGCTTTACT GAGGAATCTG ATGGACTTTC ATTGCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGAATC TGCCGAGGGC GAGATCCTCT TACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t