Construct: ORF TRCN0000479111
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013211.1_s317c1
- Derived from:
- ccsbBroadEn_14099
- DNA Barcode:
- TCGATAAGTATCGTTCATATCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECTIN3 (25945)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479111
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_017006127.2 | 29.8% | 29.6% | 1_708del;721T>C |
| 2 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_017006125.1 | 19.1% | 19% | 1_1275del;1288T>C |
| 3 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_017006124.1 | 18.8% | 18.7% | 1_1299del;1312T>C |
| 4 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | NM_015480.3 | 18.3% | 18.2% | 1_1344del;1357T>C |
| 5 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_011512663.1 | 17.3% | 17.2% | 1_1437del;1450T>C |
| 6 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XR_924122.2 | 12.7% | 1_1667del;1680T>C;1971_2368del | |
| 7 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XR_002959508.1 | 10.8% | 1_1625del;1638T>C;1929_2772del | |
| 8 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | NM_021495.4 | 16.3% | 17.1% | (many diffs) |
| 9 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_384583.3 | 7.7% | (many diffs) | |
| 10 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_001781847.1 | 2.7% | (many diffs) | |
| 11 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_001781848.1 | 2.7% | (many diffs) | |
| 12 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_001781846.1 | 2.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 393
- ORF length:
- 303
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCAAAAAGA ACCACAAATA GATGTTCTTC 121 AACAAGATGA GCTTGATTCT TACCCAGACA GTGTAAAAAA AGAAAACAAA AATCCAGTGA 181 ACAATCTAAT ACGTAAAGAC TATTTAGAAG AGCCTGAAAA AACTCAGTGG AACAATGTAG 241 AAAATCTCAA TAGGTTTGAA AGACCAATGG ATTATTATGA AGATCTAAAA ATGGGAATGA 301 AGTTTGTCAG TGATGAACAT TATGATGAAA ACGAAGATGA CTTAGTTTCA CATGTAGATG 361 GTTCCGTAAT TTCCAGGAGG GAGTGGTATG TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 541 GATAAGTATC GTTCATATCG ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt