Construct: ORF TRCN0000479114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006657.1_s317c1
- Derived from:
- ccsbBroadEn_03142
- DNA Barcode:
- CCCACCTGTCTACACTACCCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRM2B (50484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479114
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50484 | RRM2B | ribonucleotide reductase re... | NM_015713.5 | 100% | 100% | |
| 2 | human | 50484 | RRM2B | ribonucleotide reductase re... | NM_001172478.2 | 85.1% | 85.1% | 45_46ins156 |
| 3 | human | 50484 | RRM2B | ribonucleotide reductase re... | NM_001172477.1 | 82.2% | 79% | (many diffs) |
| 4 | mouse | 382985 | Rrm2b | ribonucleotide reductase M2... | NM_199476.1 | 90.5% | 93.4% | (many diffs) |
| 5 | mouse | 382985 | Rrm2b | ribonucleotide reductase M2... | XM_006520101.2 | 86.9% | 90.3% | (many diffs) |
| 6 | mouse | 382985 | Rrm2b | ribonucleotide reductase M2... | XM_006520102.3 | 77.7% | 82.3% | (many diffs) |
| 7 | mouse | 382985 | Rrm2b | ribonucleotide reductase M2... | XM_011245361.2 | 51.3% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1122
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgacccg gaaaggccgg aagcggccgg gctggatcag gatgagagat 121 catcttcaga caccaacgaa agtgaaataa agtcaaatga agagccactc ctaagaaaga 181 gttctcgccg gtttgtcatc tttccaatcc agtaccctga tatttggaaa atgtataaac 241 aggcacaggc ttccttctgg acagcagaag aggtcgactt atcaaaggat ctccctcact 301 ggaacaagct taaagcagat gagaagtact tcatctctca catcttagcc ttttttgcag 361 ccagtgatgg aattgtaaat gaaaatttgg tggagcgctt tagtcaggag gtgcaggttc 421 cagaggctcg ctgtttctat ggctttcaaa ttctcatcga gaatgttcac tcagagatgt 481 acagtttgct gatagacact tacatcagag atcccaagaa aagggaattt ttatttaatg 541 caattgaaac catgccctat gttaagaaaa aagcagattg ggccttgcga tggatagcag 601 atagaaaatc tacttttggg gaaagagtgg tggcctttgc tgctgtagaa ggagttttct 661 tctcaggatc ttttgctgct atattctggc taaagaagag aggTCTTATG CCAGGACTCA 721 CTTTTTCCAA TGAACTCATC AGCAGAGATG AAGGACTTCA CTGTGACTTT GCTTGCCTGA 781 TGTTCCAATA CTTAGTAAAT AAGCCTTCAG AAGAAAGGGT CAGGGAGATC ATTGTTGATG 841 CTGTCAAAAT TGAGCAGGAG TTTTTAACAG AAGCCTTGCC AGTTGGCCTC ATTGGAATGA 901 ATTGCATTTT GATGAAACAG TACATTGAGT TTGTAGCTGA CAGATTACTT GTGGAACTTG 961 GATTCTCAAA GGTTTTTCAG GCAGAAAATC CTTTTGATTT TATGGAAAAC ATTTCTTTAG 1021 AAGGAAAAAC AAATTTCTTT GAGAAACGAG TTTCAGAGTA TCAGCGTTTT GCAGTTATGG 1081 CAGAAACCAC AGATAACGTC TTCACCTTGG ATGCAGATTT TTTGCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGACCC ACCTGTCTAC ACTACCCGAT CACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t