Construct: ORF TRCN0000479152

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017014.1_s317c1
Derived from:
ccsbBroadEn_09240
DNA Barcode:
AACTGGGTGCCTGGCGGCGAATCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG10 (84920)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479152
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84920 ALG10 ALG10 alpha-1,2-glucosyltra... NM_032834.4 99.9% 99.7% 1416G>A
2 human 144245 ALG10B ALG10 alpha-1,2-glucosyltra... NM_001013620.4 97.6% 95.7% (many diffs)
3 human 84920 ALG10 ALG10 alpha-1,2-glucosyltra... XM_024449230.1 87.2% 87.1% 0_1ins180;1236G>A
4 human 84920 ALG10 ALG10 alpha-1,2-glucosyltra... XM_024449231.1 87.2% 87.1% 0_1ins180;1236G>A
5 human 144245 ALG10B ALG10 alpha-1,2-glucosyltra... XM_005268665.4 85.3% 83.2% (many diffs)
6 human 144245 ALG10B ALG10 alpha-1,2-glucosyltra... NM_001308340.2 25.5% 24.9% (many diffs)
7 mouse 380959 Alg10b asparagine-linked glycosyla... NM_001033441.3 82.8% 85.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1485
ORF length:
1419
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcagctggaa ggttactatt tctcggccgc cttgagctgt acctttttag
121tatcctgcct cctcttctcc gccttcagcc gggcgttgcg agagccctac atggacgaga
181tcttccacct gcctcaggcg cagcgctact gtgagggcca tttctccctt tcccagtggg
241atcccatgat tactacatta cctggcttgt acctggtgtc aattggagtg atcaaacctg
301ccatttggat ctttggatgg tctgaacatg ttgtctgctc cattgggatg ctcagatttg
361ttaatcttct cttcagtgtt ggcaacttct atttactata tttgcttttc tgcaaggtac
421aacccagaaa caaggctgcc tcaagtatcc agagagtctt gtcaacatta acactagcag
481tatttccaac actttatttt tttaacttcc tttattatac agaagcagga tctatgtttt
541ttactctttt tgcgtatttg atgtgtcttt atggaaatca taaaacttca gccttccttg
601gattttgtgg cttcatgttt cggcaaacaa atatcatctg ggctgtcttc tgtgcaggaa
661atgtcattgc acaaaagtta acggaggctt ggaaaactga gctacaaaag aaggaagaca
721gacttccacc tattaaagga ccatttgcag aattcagaaa aattcttcag tttcttttgg
781cttattccat gtcctttaaa aacttgagta tgcttttgct tctgacttgg ccctacatcc
841ttctgggatt tctgttttgt gcttttgtag tagttaatgg tggaattgtt attggcgatc
901ggagtagtca tgaagcctgt cttcattttc ctcaactatt ctactttttt tcatttactc
961tctttttttc ctttcctcat ctcctgtctc ctagcaaaat taagactttt ctttccttag
1021tttggaaacg tagaattctg ttttttgtgg ttaccttagt ctctgtgttt ttagtttGGA
1081AATTCACTTA TGCTCATAAA TACTTGCTAG CAGACAATAG ACATTATACT TTCTATGTGT
1141GGAAAAGAGT TTTTCAAAGA TATGAAACTG TAAAATATTT GTTAGTTCCA GCCTATATAT
1201TTGCTGGTTG GAGTATAGCT GACTCATTGA AATCAAAGTC AATTTTTTGG AATTTAATGT
1261TTTTCATATG CTTGTTCACT GTTATAGTTC CTCAGAAACT GCTGGAATTT CGTTACTTCA
1321TTTTACCTTA TGTCATTTAT AGGCTTAACA TACCTCTGCC TCCCACATCC AGACTCATTT
1381GTGAACTGAG CTGCTATGCA GTTGTTAATT TCATAACTTT TTTCATCTTT CTGAACAAGA
1441CTTTTCAGTG GCCAAATAGT CAGGACATTC AAAGGTTTAT ATGGTACCCA ACTTTCTTGT
1501ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1561AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1621GAAAGGACGA AACTGGGTGC CTGGCGGCGA ATCAACGCGT TAAGTCgaca atcaacctct
1681ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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