Construct: ORF TRCN0000479155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013147.1_s317c1
- Derived from:
- ccsbBroadEn_00048
- DNA Barcode:
- CTTTTATCGGACCTTAAACTAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1A1 (216)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 216 | ALDH1A1 | aldehyde dehydrogenase 1 fa... | NM_000689.5 | 100% | 100% | |
| 2 | mouse | 11668 | Aldh1a1 | aldehyde dehydrogenase fami... | NM_013467.3 | 84.5% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atcctcaggc acgccagact tacctgtcct actcaccgat ttgaagattc 121 aatatactaa gatcttcata aacaatgaat ggcatgattc agtgagtggc aagaaatttc 181 ctgtctttaa tcctgcaact gaggaggagc tctgccaggt agaagaagga gataaggagg 241 atgttgacaa ggcagtgaag gccgcaagac aggcttttca gattggatcc ccgtggcgta 301 ctatggatgc ttccgagagg gggcgactat tatacaagtt ggctgattta atcgaaagag 361 atcgtctgct gctggcgaca atggagtcaa tgaatggtgg aaaactctat tccaatgcat 421 atctgaatga tttagcaggc tgcatcaaaa cattgcgcta ctgtgcaggt tgggctgaca 481 agatccaggg ccgtacaata ccaattgatg gaaatttttt tacatataca agacatgaac 541 ctattggtgt atgtggccaa atcattcctt ggaatttccc gttggttatg ctcatttgga 601 agatagggcc tgcactgagc tgtggaaaca cagtggttgt caaaccagca gagcaaactc 661 ctctcactgc tctccacgtg gcatctttaa taaaagaggc agggtttcct cctggagtag 721 tgaatattgt tcctggttat gggcctacag caggggcagc catttcttct cacatggata 781 tagacaaagt agccttcaca ggatcaacag aggttggcaa gttgatcaaa gaagctgccg 841 ggaaaagcaa tctgaagagg gtgaccctgg agcttggagg aaagagccct tgcattgtgt 901 tagctgatgc cgacttggac aatgctgttg aatttgcaca ccatggggta ttctaccacc 961 agggccagtg ttgtatagcc gcatccagga tttttgtgga agaatcaatt tatgatgagt 1021 ttgttcgaag gagtgttgag cgggctaaga agtatatcct tggaaatcct ctgaccccag 1081 gagtcactca aggccctcag attgacaagg aacaatatga taaaatactt gaccTCATTG 1141 AGAGTGGGAA GAAAGAAGGG GCCAAACTGG AATGTGGAGG AGGCCCGTGG GGGAATAAAG 1201 GCTACTTTGT CCAGCCCACA GTGTTCTCTA ATGTTACAGA TGAGATGCGC ATTGCCAAAG 1261 AGGAGATTTT TGGACCAGTG CAGCAAATCA TGAAGTTTAA ATCTTTAGAT GACGTGATCA 1321 AAAGAGCAAA CAATACTTTC TATGGCTTAT CAGCAGGAGT GTTTACCAAA GACATTGATA 1381 AAGCCATAAC AATCTCCTCT GCTCTGCAGG CAGGAACAGT GTGGGTGAAT TGCTATGGCG 1441 TGGTAAGTGC CCAGTGCCCC TTTGGTGGAT TCAAGATGTC TGGAAATGGA AGAGAACTGG 1501 GAGAGTACGG TTTCCATGAA TATACAGAGG TCAAAACAGT CACAGTGAAA ATCTCTCAGA 1561 AGAACTCATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTTTA TCGGACCTTA AACTAACTAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt