Construct: ORF TRCN0000479159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002501.1_s317c1
- Derived from:
- ccsbBroadEn_12878
- DNA Barcode:
- AGCCCGTGTTGGATTTCAGTAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFATC2IP (84901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84901 | NFATC2IP | nuclear factor of activated... | NM_032815.3 | 30.3% | 30.3% | 1_876del |
| 2 | human | 84901 | NFATC2IP | nuclear factor of activated... | XR_950864.3 | 14.7% | 1_628del;1010_2585del | |
| 3 | mouse | 18020 | Nfatc2ip | nuclear factor of activated... | NM_010900.3 | 26.6% | 27.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 471
- ORF length:
- 381
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCACCCA CCTTGGGGTG TCCCCAAGCA 121 GGATCCTTTT GCTTTTTGGA GAGACAGAGC TATCACCTAC TGCCACTCCC AGGACCCTAA 181 AGCTCGGAGT GGCTGACATC ATTGACTGTG TGGTACTAAC AAGTTCTCCA GAGGCCACAG 241 AGACGTCCCA ACAGCTCCAG CTCCGGGTGC AGGGAAAGGA GAAACACCAG ACACTGGAAG 301 TCTCACTGTC TCGAGATTCC CCTCTAAAGA CCCTCATGTC CCACTATGAG GAGGCCATGG 361 GACTGTCGGG ACGGAAGCTC TCCTTCTTCT TTGATGGGAC AAAGCTTTCA GGCAGGGAGC 421 TGCCAGCTGA CCTGGGCATG GAATCTGGGG ACCTCATTGA GGTCTGGGGC TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGCATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAAGCC CGTGTTGGAT TTCAGTAACT ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt