Construct: ORF TRCN0000479169
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004499.1_s317c1
- Derived from:
- ccsbBroadEn_01022
- DNA Barcode:
- AGTCGACTTAATGACCAACCTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MMP12 (4321)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479169
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4321 | MMP12 | matrix metallopeptidase 12 | NM_002426.6 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1479
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagtttctt ctaatactgc tcctgcaggc cactgcttct ggagctcttc 121 ccctgaacag ctctacaagc ctggaaaaaa ataatgtgct atttggtgaa agatacttag 181 aaaaatttta tggccttgag ataaacaaac ttccagtgac aaaaatgaaa tatagtggaa 241 acttaatgaa ggaaaaaatc caagaaatgc agcacttctt gggtctgaaa gtgaccgggc 301 aactggacac atctaccctg gagatgatgc acgcacctcg atgtggagtc cccgatgtcc 361 atcatttcag ggaaatgcca ggggggcccg tatggaggaa acattatatc acctacagaa 421 tcaataatta cacacctgac atgaaccgtg aggatgttga ctacgcaatc cggaaagctt 481 tccaagtatg gagtaatgtt acccccttga aattcagcaa gattaacaca ggcatggctg 541 acattttggt ggtttttgcc cgtggagctc atggagactt ccatgctttt gatggcaaag 601 gtggaatcct agcccatgct tttggacctg gatctggcat tggaggggat gcacatttcg 661 atgaggacga attctggact acacattcag gaggcacaaa cttgttcctc actgctgttc 721 acgagattgg ccattcctta ggtcttggcc attctagtga tccaaaggcc gtaatgttcc 781 ccacctacaa atatgttgac atcaacacat ttcgcctctc tgctgatgac atacgtggca 841 ttcagtccct gtatggagac ccaaaagaga accaacgctt gccaaatcct gacaattcag 901 aaccagctct ctgtgacccc aatttgagtt ttgatgctgt cactaccgtg ggaaataaga 961 tctttttctt caaagacagg ttcttctggc tgaaggtttc tgagagacca aagaccagtg 1021 ttaatttaat ttcttcctta tggccaacct tgccatctgg cattgaagct gcttatgaaa 1081 ttGAAGCCAG AAATCAAGTT TTTCTTTTTA AAGATGACAA ATACTGGTTA ATTAGCAATT 1141 TAAGACCAGA GCCAAATTAT CCCAAGAGCA TACATTCTTT TGGTTTTCCT AACTTTGTGA 1201 AAAAAATTGA TGCAGCTGTT TTTAACCCAC GTTTTTATAG GACCTACTTC TTTGTAGATA 1261 ACCAGTATTG GAGGTATGAT GAAAGGAGAC AGATGATGGA CCCTGGTTAT CCCAAACTGA 1321 TTACCAAGAA CTTCCAAGGA ATCGGGCCTA AAATTGATGC AGTCTTCTAC TCTAAAAACA 1381 AATACTACTA TTTCTTCCAA GGATCTAACC AATTTGAATA TGACTTCCTA CTCCAACGTA 1441 TCACCAAAAC ACTGAAAAGC AATAGCTGGT TTGGTTGTTT GCCAACTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGAAGTCGA CTTAATGACC AACCTAAAAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt