Construct: ORF TRCN0000479173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016034.1_s317c1
- Derived from:
- ccsbBroadEn_08347
- DNA Barcode:
- CCGATACATACGAAAACCTTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT7 (51809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51809 | GALNT7 | polypeptide N-acetylgalacto... | NM_017423.3 | 99.9% | 100% | 18G>A |
| 2 | human | 51809 | GALNT7 | polypeptide N-acetylgalacto... | XM_005263062.2 | 97% | 97.5% | (many diffs) |
| 3 | human | 51809 | GALNT7 | polypeptide N-acetylgalacto... | XM_017008292.2 | 96% | 92.6% | (many diffs) |
| 4 | human | 51809 | GALNT7 | polypeptide N-acetylgalacto... | XM_011532025.3 | 93.7% | 93.6% | 0_1ins123;2T>G |
| 5 | human | 51809 | GALNT7 | polypeptide N-acetylgalacto... | XM_017008293.1 | 93.1% | 90.2% | (many diffs) |
| 6 | mouse | 108150 | Galnt7 | UDP-N-acetyl-alpha-D-galact... | NM_144731.4 | 91.3% | 95.1% | (many diffs) |
| 7 | mouse | 108150 | Galnt7 | UDP-N-acetyl-alpha-D-galact... | NM_001167981.1 | 88.7% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2037
- ORF length:
- 1971
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gctgaagatt ggattcatct tacgcagttt gctggtggtg ggaagcttcc 121 tggggctagt ggtcctctgg tcttccctga ccccgcggcc ggacgaccca agcccgctga 181 gcaggatgag ggaagacaga gatgtcaatg accccatgcc caaccgaggc ggcaatggac 241 tagctcctgg ggaggacaga ttcaaacctg tggtaccatg gcctcatgtt gaaggagtag 301 aagtggactt agagtctatt agaagaataa acaaggccaa aaatgaacaa gagcaccatg 361 ctggaggaga ttcccagaaa gatatcatgc agaggcagta tctcacattt aagcctcaga 421 cattcaccta ccatgatcct gtgcttcgcc cagggatcct cggtaacttt gaacccaaag 481 aacctgagcc tcctggagtg gttggtggcc ctggagagaa agccaagcca ttggttttgg 541 gaccagaatt caaacaagca attcaagcca gcattaaaga gtttggattt aacatggtgg 601 caagtgacat gatctcactg gaccgcagcg tcaatgactt acgccaagaa gaatgcaagt 661 attggcatta tgatgaaaac ttgctcactt cgagcgttgt cattgtcttc cataatgaag 721 gatggtcaac cctcatgaga acagtccaca gtgtaattaa aaggactcca aggaaatatt 781 tagcagaaat tgtgttaatt gacgatttca gtaataaaga acacttaaaa gaaaaactgg 841 atgaatatat taagctgtgg aatggcctag tgaaggtatt tcgaaatgaa agaagggaag 901 gtttaattca agcacgaagt attggtgctc agaaggctaa acttggacag gttttgatat 961 accttgatgc ccactgtgag gtggcagtta actggtatgc accacttgta gctcccatat 1021 ctaaggacag aaccatttgc actgtgccgc ttatagatgt cataaatggc aacacatatg 1081 aaattatacc ccaagggggt ggtgatgaag atgggtatgc ccgaggagca tgggattgga 1141 gtatgctctg gaaacgggtg cctctgaccc ctcaagagaa gagactgaga aagacaaaaa 1201 ctgaaccgta tcggtcccca gccatggctg ggggattatt tgccattgaa cgagagttct 1261 tctttgaatt gggtctctat gatccaggtc tccagatttg gggtggtgaa aactttgaga 1321 tctcatacaa gatatggcag tgtggtggca aattattatt tgttccttgt tctcgtgttg 1381 gacatatcta ccgtcttgag ggctggcaag gaaatcctcc gcccatttat gttgggtctt 1441 ctccaactct gaagaattat gttagagttg tggaggtttg gtgggatgaa tataaagact 1501 acttctatgc tagtcgtcct gaatcgcagg cattaccata tggggatata tcggagctga 1561 aaaaatttcg agaagatcac aactgcaaaa gttttaagtg gttcatggaa gaaatagctt 1621 atgatatcac ctcacactac cctttgccac ccaaaaatgt tgactgggga gaaatcagag 1681 gcttcgaaac tgcttactgc attGATAGCA TGGGAAAAAC AAATGGAGGC TTTGTTGAAC 1741 TAGGACCCTG CCACAGGATG GGAGGGAATC AGCTTTTCAG AATCAATGAA GCAAATCAAC 1801 TCATGCAGTA TGACCAGTGT TTGACAAAGG GAGCTGATGG ATCAAAAGTT ATGATTACAC 1861 ACTGTAATCT AAATGAATTT AAGGAATGGC AGTACTTCAA GAACCTGCAC AGATTTACTC 1921 ATATTCCTTC AGGAAAGTGT TTAGATCGCT CAGAGGTCCT GCATCAAGTA TTCATCTCCA 1981 ATTGTGACTC CAGTAAAACG ACTCAAAAAT GGGAAATGAA TAACATCCAT AGTGTTTACC 2041 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2101 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2161 ATATATCTTG TGGAAAGGAC GACCGATACA TACGAAAACC TTCCGGACGC GTTAAGTCga 2221 caatcaacct ctggattaca aaatttgtga aagatt