Construct: ORF TRCN0000479182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017271.1_s317c1
- Derived from:
- ccsbBroadEn_01075
- DNA Barcode:
- ATACACTATCAGGCATGCCTCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFV1 (4723)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4723 | NDUFV1 | NADH:ubiquinone oxidoreduct... | NM_007103.4 | 100% | 100% | |
| 2 | human | 4723 | NDUFV1 | NADH:ubiquinone oxidoreduct... | NM_001166102.2 | 98% | 98% | 44_45ins27 |
| 3 | mouse | 17995 | Ndufv1 | NADH dehydrogenase (ubiquin... | NM_133666.3 | 89.7% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggcaacacgg cggctgctcg gctggtcgct tcccgcgcgg gtatctgtgc 121 gtttcagcgg cgacacgaca gcacccaaga aaacctcatt tggctcgctg aaggatgaag 181 accggatttt caccaacctg tacggccgcc atgactggag gctgaaaggt tccctgagtc 241 gaggtgactg gtacaagaca aaggagatcc tgctgaaggg gcccgactgg atcctgggcg 301 agatcaagac atcgggtttg aggggccgtg gaggcgctgg cttccccact ggcctcaagt 361 ggagcttcat gaataagccc tcagatggca ggcccaagta tctggtggtg aacgcagacg 421 agggggagcc gggcacctgc aaggaccggg agatcttacg ccatgatcct cacaagctgc 481 tggaaggctg cctggtgggg ggccgggcca tgggcgcccg cgctgcctat atctacatcc 541 gaggggaatt ctacaatgag gcctccaatc tgcaggtggc catccgagag gcctatgagg 601 caggtctgat tggcaagaat gcttgtggct ctggctatga ttttgacgtg tttgtggtgc 661 gcggggctgg ggcctacatc tgtggagagg agacagcgct catcgagtcc attgagggca 721 agcagggcaa gccccgcctg aagcccccct tccccgcaga cgtgggagtg tttggctgcc 781 ccacaactgt ggccaacgtg gagacagtgg cagtgtcccc cacaatctgc cgccgtggag 841 gtacctggtt tgctggcttt ggcagagaac gcaactcagg caccaaacta ttcaacatct 901 ctggccatgt caaccaccct tgcactgtgg aggaggagat gtctgtgccc ttgaaagaac 961 tgattgagaa gcatgctggg ggtgtcacgg gcggctggga caacctcctt gctgtgatcc 1021 ctggcggctc GTCTACCCCA CTGATCCCCA AGTCTGTGTG TGAGACGGTG CTGATGGACT 1081 TCGATGCGCT GGTGCAGGCA CAGACAGGCC TGGGCACAGC TGCGGTGATC GTCATGGACC 1141 GCTCGACGGA CATCGTGAAA GCCATCGCCC GCCTCATTGA GTTCTATAAG CACGAGAGCT 1201 GTGGCCAGTG TACCCCATGC CGTGAGGGTG TGGACTGGAT GAACAAGGTG ATGGCACGTT 1261 TCGTGAGGGG GGATGCCCGG CCGGCCGAGA TCGACTCCCT GTGGGAGATC AGCAAGCAGA 1321 TAGAAGGCCA TACGATTTGT GCTCTGGGTG ACGGGGCCGC CTGGCCTGTG CAGGGTCTGA 1381 TCCGCCACTT TCGGCCGGAG CTCGAGGAGC GGATGCAGCG GTTTGCCCAG CAGCATCAGG 1441 CCCGGCAGGC TGCCTCTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATACACT ATCAGGCATG 1621 CCTCGTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt