Construct: ORF TRCN0000479197
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015251.1_s317c1
- Derived from:
- ccsbBroadEn_08273
- DNA Barcode:
- CCCCTTCGGAAAGTGATGACCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP39A1 (51302)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479197
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | NM_016593.5 | 99.8% | 99.5% | 68G>C;862T>C |
| 2 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | NM_001278738.1 | 95.5% | 95.3% | 68G>C;311_312ins60;802T>C |
| 3 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_017010924.1 | 92.1% | 91.8% | 68G>C;732_733ins108;754T>C |
| 4 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_017010921.1 | 90.2% | 86.2% | (many diffs) |
| 5 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_005249171.4 | 88.7% | 88.5% | (many diffs) |
| 6 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_017010922.1 | 86.1% | 82.1% | (many diffs) |
| 7 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_017010923.1 | 82.8% | 78.9% | (many diffs) |
| 8 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_011514658.1 | 78.9% | 75.3% | (many diffs) |
| 9 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_024446452.1 | 76.4% | 72.6% | (many diffs) |
| 10 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_017010925.1 | 71.2% | 67.6% | (many diffs) |
| 11 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | XM_024446453.1 | 65.6% | 61.8% | (many diffs) |
| 12 | human | 51302 | CYP39A1 | cytochrome P450 family 39 s... | NM_001278739.1 | 63.2% | 63.1% | 0_1ins516;346T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga actaatttcc ccaacagtga ttataatcct gggttgcctt gctctgttct 121 tactccttca gccgaagaat ttgcgtagac ccccgtgcat caagggctgg attccttgga 181 ttggagttgg atttgagttt gggaaagccc ctctagaatt tatagagaaa gcaagaatca 241 agtatggacc aatatttaca gtctttgcta tgggaaaccg aatgaccttt gttactgaag 301 aagaaggaat taatgtgttt ctaaaatcca aaaaagtaga ttttgaacta gcagtgcaaa 361 atatcgttta tcgtacagca tcaattccaa agaatgtctt tttagcactg catgaaaaac 421 tctatattat gttgaaaggg aaaatgggga ctgtcaatct ccatcagttt actgggcaac 481 tgactgaaga attacatgaa caactggaga atttaggcac tcatgggaca atggacctga 541 acaacttagt aagacatctc ctttatccag tcacagtgaa tatgctcttt aataaaagtt 601 tgttttccac aaacaagaaa aaaatcaagg agttccatca gtattttcaa gtttatgatg 661 aagattttga gtatgggtcc cagttgccag agtgtcttct aagaaactgg tcaaaatcca 721 aaaagtggtt cctggaactg tttgagaaaa acattccaga tataaaagca tgtaaatctg 781 caaaagataa ttccatgaca ttattgcaag ctacgctgga tattgtagag acggaaacaa 841 gtaaggaaaa ctcacccaat tatgggctct tactgctttg ggcttctctg tctaatgctg 901 ttcctgttgc attttggaca cttgcacacg tcctttctca tcctgatatc cacaaggcca 961 ttatggaagg catatcttct gtgtttggca aagcaggcaa agataagatt aaagtgtctg 1021 aggatgacct ggagaatctc cttctaatta aatggtgtgt tttggaaaCC ATTCGTTTAA 1081 AAGCTCCTGG TGTCATTACT AGAAAAGTGG TGAAGCCTGT GGAAATTTTG AATTACATCA 1141 TTCCTTCTGG TGACTTGTTG ATGTTGTCTC CATTTTGGCT GCATAGAAAT CCAAAGTATT 1201 TTCCTGAGCC TGAATTGTTC AAACCTGAAC GTTGGAAAAA GGCAAATTTA GAGAAGCACT 1261 CTTTCTTGGA CTGCTTCATG GCATTTGGAA GCGGGAAGTT CCAGTGTCCT GCAAGGTGGT 1321 TTGCTCTGTT AGAGGTTCAG ATGTGTATTA TTTTAATACT TTATAAATAT GACTGTAGTC 1381 TTCTGGACCC ATTACCCAAA CAGAGTTATC TCCATTTGGT GGGTGTCCCC CAGCCGGAAG 1441 GGCAATGCCG AATTGAATAT AAACAAAGAA TATGCCCAAC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1621 CCTTCGGAAA GTGATGACCT TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt