Construct: ORF TRCN0000479213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008058.1_s317c1
- Derived from:
- ccsbBroadEn_15853
- DNA Barcode:
- CACCGACTAAAGGCCTGTCTACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC2LI1 (51626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_015522.3 | 67.9% | 57.2% | (many diffs) |
| 2 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_016008.4 | 39.5% | 32.7% | (many diffs) |
| 3 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001193464.2 | 39.4% | 32.6% | (many diffs) |
| 4 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001348912.1 | 37.1% | 30.8% | (many diffs) |
| 5 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001348913.1 | 37% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 522
- ORF length:
- 432
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCAGTGA AACTCTCTGG GAAATTGCAA 121 AAGCTGAAGT GGAAAAAAGG GGAATTAATG GAAGTGAAGG TGATGGAGCT GAAATTGCAG 181 AAAAATTTGT TTTCTTCATT GGCAGTAAAA ATGGGGGAAA GACTACTATT ATTCTAAGGT 241 GTCTTGACAG AGATGAACCA CCAAAACCAA CCTTAGCTTT GGAATATACA TATGGAAGAA 301 GAGCAAAAGG GCACAACACA CCAAAAGATA TCGCTCACTT TTGGGAACTC GGTGGAGGAA 361 CCTCTTTATT GGACTTAATC AGCATACCCA TCACAGGTGA CACCTTACGC TCCTGGCAAC 421 TATCATCCCT ACTGCCTGTC TCTATGAATT TGACCACTCC AGTACCTCAT ATAAATGAAA 481 TCATTCATTA TTTGTCCTTT TGTTATTTAT TTCATTTAGC ATACCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGACAC CGACTAAAGG CCTGTCTACT TACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t