Construct: ORF TRCN0000479242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017846.1_s317c1
- Derived from:
- ccsbBroadEn_00825
- DNA Barcode:
- AGCGATAAGTTTAAGGCCAGGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA6 (3443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 100% | 100% | |
| 2 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 91% | 86.2% | (many diffs) |
| 3 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 90.8% | 84.6% | (many diffs) |
| 4 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 90.5% | 84.7% | (many diffs) |
| 5 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 90.2% | 85.7% | (many diffs) |
| 6 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 90.1% | 83% | (many diffs) |
| 7 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 89.6% | 81.4% | (many diffs) |
| 8 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 89% | 81.4% | (many diffs) |
| 9 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 88.7% | 79.8% | (many diffs) |
| 10 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 88.5% | 80.9% | (many diffs) |
| 11 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 88.3% | 80.9% | (many diffs) |
| 12 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 88% | 79.8% | (many diffs) |
| 13 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 87.8% | 77.7% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 47.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctttgcct tttgctttac tgatggccct ggtggtgctc agctgcaagt 121 caagctgctc tctggactgt gatctgcctc agacccacag cctgggtcac aggaggacca 181 tgatgctcct GGCACAAATG AGGAGAATCT CTCTTTTCTC CTGTCTGAAG GACAGACATG 241 ACTTCAGATT TCCCCAGGAG GAGTTTGATG GCAACCAGTT CCAGAAGGCT GAAGCCATCT 301 CTGTCCTCCA TGAGGTGATT CAGCAGACCT TCAACCTCTT CAGCACAAAG GACTCATCTG 361 TTGCTTGGGA TGAGAGGCTT CTAGACAAAC TCTATACTGA ACTTTACCAG CAGCTGAATG 421 ACCTGGAAGC CTGTGTGATG CAGGAGGTGT GGGTGGGAGG GACTCCCCTG ATGAATGAGG 481 ACTCCATCCT GGCTGTGAGA AAATACTTCC AAAGAATCAC TCTCTACCTG ACAGAGAAAA 541 AGTACAGCCC TTGTGCCTGG GAGGTTGTCA GAGCAGAAAT CATGAGATCC TTCTCTTCAT 601 CAAGAAACTT GCAAGAAAGG TTAAGGAGGA AGGAATTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AAGCGATAAG TTTAAGGCCA GGATAACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt