Construct: ORF TRCN0000479246
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000957.1_s317c1
- Derived from:
- ccsbBroadEn_07796
- DNA Barcode:
- ATCACGCCCTACTTCTCGTCGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD300A (11314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479246
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11314 | CD300A | CD300a molecule | NM_007261.4 | 99.8% | 99.6% | 890G>C |
| 2 | human | 11314 | CD300A | CD300a molecule | XM_005256990.3 | 87.8% | 87.6% | 665_666ins108;782G>C |
| 3 | human | 11314 | CD300A | CD300a molecule | XM_005256991.5 | 74.5% | 67.7% | 627_628ins146;669_670ins82 |
| 4 | human | 11314 | CD300A | CD300a molecule | NM_001256841.2 | 60.9% | 61.8% | (many diffs) |
| 5 | human | 11314 | CD300A | CD300a molecule | NM_001330456.1 | 56.4% | 56.1% | 0_1ins390;500G>C |
| 6 | human | 11314 | CD300A | CD300a molecule | XM_006721656.3 | 56.4% | 56.1% | 0_1ins390;500G>C |
| 7 | human | 11314 | CD300A | CD300a molecule | NM_001330457.2 | 48.8% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gctgccttgg gctctgttgc ttctctgggt cccaggatgt tttgctctga 121 gcaaatgcag gaccgtggcg ggccccgtgg ggggatccct gagtgtgcag tgtccctatg 181 agaaggaaca caggaccctc aacaaatact ggtgcagacc accacagatt ttcctatgtg 241 acaagattgt ggagaccaaa gggtcagcag gaaaaaggaa cggccgagtg tccatcaggg 301 acagtcctgc aaacctcagc ttcacagtga ccctggagaa tctcacagag gaggatgcag 361 gcacctactg gtgtggggtg gatacaccat ggctccgaga ctttcatgat cccgttgtcg 421 aggttgaggt gtccgtgttc ccggcatcaa cgtcaatgac acctgcaagt atcactgcgg 481 ccaagacctc aacaatcaca actgcatttc cacctgtatc atccactacc ctgtttgcag 541 tgggtgccac ccacagtgcc agcatccagg aggaaactga ggaggtggtg aactcacagc 601 tcccgcTGCT CCTCTCCCTG CTGGCATTGT TGCTGCTTCT GTTGGTGGGG GCCTCCCTGC 661 TAGCCTGGAG GATGTTTCAG AAATGGATCA AAGCTGGTGA CCATTCAGAG CTGTCCCAGA 721 ACCCCAAGCA GGCTGCCACG CAGAGTGAGC TGCACTACGC AAATCTGGAG CTGCTGATGT 781 GGCCTCTGCA GGAAAAGCCA GCACCACCAA GGGAGGTGGA GGTGGAATAC AGCACTGTGG 841 CCTCCCCCAG GGAAGAACTT CACTATGCCT CGGTGGTGTT TGATTCTAAC ACCAACAGGA 901 TAGCTGCTCA GAGGCCTCGG GAGGAGGAAC CAGATTCAGA TTACAGTGTG ATAACGAAGA 961 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGAAT CACGCCCTAC TTCTCGTCGC GGACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt