Construct: ORF TRCN0000479247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000451.2_s317c1
- Derived from:
- ccsbBroadEn_11178
- DNA Barcode:
- CTATAAGAGCTCAATCACTGCGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCN1 (6947)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6947 | TCN1 | transcobalamin 1 | NM_001062.4 | 97.6% | 97.6% | 846C>T;1270_1299del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag acagtcacac cagctgcccc tagtggggct cttactgttt tcttttattc 121 caagccaact atgcgagatt tgtgaggtaa gtgaagaaaa ctacatccgc ctaaaacctc 181 tgttgaatac aatgatccag tcaaactata acaggggaac cagcgctgtc aatgttgtgt 241 tgtccctcaa acttgttgga atccagatcc aaaccctgat gcaaaagatg atccaacaaa 301 tcaaatacaa tgtgaaaagc agattgtcag atgtaagctc gggagagctt gccttgatta 361 tactggcttt gggagtatgt cgtaacgctg aggaaaactt aatatatgat taccacctga 421 tcgacaagct agaaaataaa ttccaagcag aaattgaaaa tatggaagca cacaatggca 481 ctcccctgac taactactac cagctcagcc tggacgtttt ggccttgtgt ctgttcaatg 541 ggaactactc aaccgccgaa gttgtcaacc acttcactcc tgaaaataaa aactattatt 601 ttggtagcca gttctcagta gatactggtg caatggctgt cctggctctg acctgtgtga 661 agaagagtct aataaatggg cagatcaaag cagatgaagg cagtttaaag aacatcagta 721 tttatacaaa gtcactggta gaaaagattc tgtctgagaa aaaagaaaat ggtctcattg 781 gaaacacatt tagcacagga gaagccatgc aggccctctt tgtatcatca gactattata 841 atgaaaatga ctggaattgc caacaaactc tgaatacagt gctcacggaa atttctcaag 901 gagcattcag taatccaaac gctgcagccc aggtcttacc tgccctgatg ggaaagacct 961 tcttggatat taacaaagac tcTTCTTGCG TCTCTGCTTC AGGTAACTTC AACATCTCCG 1021 CTGATGAGCC TATAACTGTG ACACCTCCTG ACTCACAATC ATATATCTCC GTCAATTACT 1081 CTGTGAGAAT CAATGAAACA TATTTCACCA ATGTCACTGT GCTAAATGGT TCTGTCTTCC 1141 TCAGTGTGAT GGAGAAAGCC CAGAAAATGA ATGATACTAT ATTTGGTTTC ACAATGGAGG 1201 AGCGCTCATG GGGGCCCTAT ATCACCTGTA TTCAGGGCCT ATGTGCCAAC AATAATGACA 1261 GAACCTACTG GGAACTTCTG AGTGGAGGCG AACCACTGAG CCAAGGAGCT GGTAGTTACG 1321 TTGTCCGCAA TGGATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTATAAGAGC TCAATCACTG 1501 CGGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt