Transcript: Human NM_001062.4

Homo sapiens transcobalamin 1 (TCN1), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
TCN1 (6947)
Length:
1486
CDS:
18..1319

Additional Resources:

NCBI RefSeq record:
NM_001062.4
NBCI Gene record:
TCN1 (6947)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001062.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000373596 CTCCGCTGATGAGCCTATAAC pLKO_005 968 CDS 100% 13.200 18.480 N TCN1 n/a
2 TRCN0000059980 CGTCTCTGCTTCAGGTAACTT pLKO.1 941 CDS 100% 5.625 7.875 N TCN1 n/a
3 TRCN0000059978 CGTCAATTACTCTGTGAGAAT pLKO.1 1022 CDS 100% 4.950 6.930 N TCN1 n/a
4 TRCN0000373597 GAAAGACCTTCTTGGATATTA pLKO_005 904 CDS 100% 15.000 10.500 N TCN1 n/a
5 TRCN0000373663 CAAGCCAACTATGCGAGATTT pLKO_005 73 CDS 100% 13.200 9.240 N TCN1 n/a
6 TRCN0000059981 CCTCTTTGTATCATCAGACTA pLKO.1 767 CDS 100% 4.950 3.465 N TCN1 n/a
7 TRCN0000059982 CTCAGTAGATACTGGTGCAAT pLKO.1 566 CDS 100% 4.950 3.465 N TCN1 n/a
8 TRCN0000378992 ATACAGTGCTCACGGAAATTT pLKO_005 826 CDS 100% 15.000 9.000 N TCN1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001062.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11178 pDONR223 100% 97.6% 97.6% None 846C>T;1270_1299del n/a
2 ccsbBroad304_11178 pLX_304 0% 97.6% 97.6% V5 846C>T;1270_1299del n/a
3 TRCN0000479247 CTATAAGAGCTCAATCACTGCGGA pLX_317 28.1% 97.6% 97.6% V5 846C>T;1270_1299del n/a
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