Construct: ORF TRCN0000479270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007391.1_s317c1
- Derived from:
- ccsbBroadEn_09417
- DNA Barcode:
- CGAGAAGGACTGAAAATCGTTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GBP4 (115361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115361 | GBP4 | guanylate binding protein 4 | NM_052941.5 | 99.8% | 99.6% | 892G>A;1504G>T |
| 2 | human | 388646 | GBP7 | guanylate binding protein 7 | NM_207398.3 | 81.8% | 73.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1989
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtgagaga actcttcacg ctgcagtgcc cacaccaggt tatccagaat 121 ctgaatccat catgatggcc cccatttgtc tagtggaaaa ccaggaagag cagctgacag 181 tgaattcaaa ggcattagag attcttgaca agatttctca gcccgtggtg gtggtggcca 241 ttgtagggct ataccgcaca ggaaaatcct atctcatgaa tcgtcttgca ggaaagcgca 301 atggcttccc tctgggctcc acggtgcagt ctgaaactaa gggcatctgg atgtggtgtg 361 tgccccacct ctctaagcca aaccacaccc tggtccttct ggacaccgag ggcctgggcg 421 atgtagaaaa gagtaaccct aagaatgact cgtggatctt tgccctggct gtgcttctaa 481 gcagcagctt tgtctataac agcgtgagca ccatcaacca ccaggccctg gagcagctgc 541 actatgtgac tgagctagca gagctaatca gggcaaaatc ctgccccaga cctgatgaag 601 ctgaggactc cagcgagttt gcgagtttct ttccagactt tatttggact gttcgggatt 661 ttaccctgga gctaaagtta gatggaaacc ccatcacaga agatgagtac ctggagaatg 721 ccttgaagct gattccaggc aagaatccca aaattcaaaa ttcaaacatg cctagagagt 781 gtatcaggca tttcttccga aaacggaagt gctttgtctt tgaccggcct acaaatgaca 841 agcaatattt aaatcatatg gacgaagtgc cagaagaaaa tctggaaagg catttcctta 901 tgcaatcaga caacttctgt tcttatatct tcacccatgc aaagaccaag accctgagaa 961 agggaatcat tgtcactgga aagcggctgg ggactctggt ggtgacttat gtagatgcca 1021 tcaacagtgg agcagtacct tgtctggaga atgcagtgac agcactggcc cagcttgaga 1081 acccagcggc tgtgcagagg gcagccgacc actatagcca gcagatggcc cagcaactga 1141 ggctccccac agacacgctc caggagctgc tggacgtgca tgcagcctgt gagagggaag 1201 ccattgcagt cttcatggag cactccttca aggatgaaaa ccatgaattc cagaagaagc 1261 ttgtggacac catagagaaa aagaagggag actttgtgct gcagaatgaa gaggcatctg 1321 ccaaatattg ccaggctgag cttaagcggc tttcagagca cctgacagaa agcattttga 1381 gaggaatttt ctctgttcct ggaggacaca atctctactt agaagaaaag aaacaggttg 1441 agtgggacta taagctagtg cccagaaaag gagttaaggc aaacgaggtc ctccagaact 1501 tcctgcagtc acaggtggtt gtagaggaat ccatcctgca gtcagacaaa gccctcactg 1561 ctggagagaa gtccatagca gcggagcggg ccatgaagga agcagctgag aaggaacagg 1621 agctgcTAAG AGAAAAACAG AAGGAGCAGC AGCAAATGAT GGAGGCTCAA GAGAGAAGCT 1681 TCCAGGAATA CATGGCCCAA ATGGAGAAGA AGTTGGAGGA GGAAAGGGAA AACCTTCTCA 1741 GAGAGCATGA AAGGCTGCTA AAACACAAGC TGAAGGTACA AGAAGAAATG CTTAAGGAAG 1801 AATTTCAAAA GAAATCTGAG CAGTTAAATA AAGAGATTAA TCAACTGAAA GAAAAAATTG 1861 AAAGCACTAA AAATGAACAG TTAAGGCTCT TAAAGATCCT TGACATGGCT AGCAACATAA 1921 TGATTGTCAC TCTACCTGGG GCTTCCAAGC TACTTGGAGT AGGGACAAAA TATCTTGGCT 1981 CACGTATTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2041 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2101 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGAGAA GGACTGAAAA TCGTTAGTAC 2161 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt