Construct: ORF TRCN0000479287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000341.1_s317c1
- Derived from:
- ccsbBroadEn_05749
- DNA Barcode:
- GTCGAGGGACCAGTTCTTAGTAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- A2M (2)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479287
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2 | A2M | alpha-2-macroglobulin | NM_000014.5 | 99.9% | 99.9% | 2998A>G |
| 2 | human | 2 | A2M | alpha-2-macroglobulin | NM_001347423.2 | 99.9% | 99.8% | 1915A>G;2998A>G |
| 3 | human | 2 | A2M | alpha-2-macroglobulin | XM_006719056.3 | 97.3% | 96.9% | (many diffs) |
| 4 | human | 2 | A2M | alpha-2-macroglobulin | NM_001347424.1 | 93.1% | 93.1% | 0_1ins300;2698A>G |
| 5 | human | 2 | A2M | alpha-2-macroglobulin | NM_001347425.1 | 89.8% | 89.7% | 0_1ins450;2548A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4488
- ORF length:
- 4422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagaacaaa ctccttcatc caagtctggt tcttctcctc ttggtcctcc 121 tgcccacaga cgcctcagtc tctggaaaac cgcagtatat ggttctggtc ccctccctgc 181 tccacactga gaccactgag aagggctgtg tccttctgag ctacctgaat gagacagtga 241 ctgtaagtgc ttccttggag tctgtcaggg gaaacaggag cctcttcact gacctggagg 301 cggagaatga cgtactccac tgtgtcgcct tcgctgtccc aaagtcttca tccaatgagg 361 aggtaatgtt cctcactgtc caagtgaaag gaccaaccca agaatttaag aagcggacca 421 cagtgatggt taagaacgag gacagtctgg tctttgtcca gacagacaaa tcaatctaca 481 aaccagggca gacagtgaaa tttcgtgttg tctccatgga tgaaaacttt caccccctga 541 atgagttgat tccactagta tacattcagg atcccaaagg aaatcgcatc gcacaatggc 601 agagtttcca gttagagggt ggcctcaagc aattttcttt tcccctctca tcagagccct 661 tccagggctc ctacaaggtg gtggtacaga agaaatcagg tggaaggaca gagcaccctt 721 tcaccgtgga ggaatttgtt cttcccaagt ttgaagtaca agtaacagtg ccaaagataa 781 tcaccatctt ggaagaagag atgaatgtat cagtgtgtgg cctatacaca tatgggaagc 841 ctgtccctgg acatgtgact gtgagcattt gcagaaagta tagtgacgct tccgactgcc 901 acggtgaaga ttcacaggct ttctgtgaga aattcagtgg acagctaaac agccatggct 961 gcttctatca gcaagtaaaa accaaggtct tccagctgaa gaggaaggag tatgaaatga 1021 aacttcacac tgaggcccag atccaagaag aaggaacagt ggtggaattg actggaaggc 1081 agtccagtga aatcacaaga accataacca aactctcatt tgtgaaagtg gactcacact 1141 ttcgacaggg aattcccttc tttgggcagg tgcgcctagt agatgggaaa ggcgtcccta 1201 taccaaataa agtcatattc atcagaggaa atgaagcaaa ctattactcc aatgctacca 1261 cggatgagca tggccttgta cagttctcta tcaacaccac caatgttatg ggtacctctc 1321 ttactgttag ggtcaattac aaggatcgta gtccctgtta cggctaccag tgggtgtcag 1381 aagaacacga agaggcacat cacactgctt atcttgtgtt ctccccaagc aagagctttg 1441 tccaccttga gcccatgtct catgaactac cctgtggcca tactcagaca gtccaggcac 1501 attatattct gaatggaggc accctgctgg ggctgaagaa gctctccttc tattatctga 1561 taatggcaaa gggaggcatt gtccgaactg ggactcatgg actgcttgtg aagcaggaag 1621 acatgaaggg ccatttttcc atctcaatcc ctgtgaagtc agacattgct cctgtcgctc 1681 ggttgctcat ctatgctgtt ttacctaccg gggacgtgat tggggattct gcaaaatatg 1741 atgttgaaaa ttgtctggcc aacaaggtgg atttgagctt cagcccatca caaagtctcc 1801 cagcctcaca cgcccacctg cgagtcacag cggctcctca gtccgtctgc gccctccgtg 1861 ctgtggacca aagcgtgctg ctcatgaagc ctgatgctga gctctcggcg tcctcggttt 1921 acaacctgct accagaaaag gacctcactg gcttccctgg gcctttgaat gaccaggacg 1981 atgaagactg catcaatcgt cataatgtct atattaatgg aatcacatat actccagtat 2041 caagtacaaa tgaaaaggat atgtacagct tcctagagga catgggctta aaggcattca 2101 ccaactcaaa gattcgtaaa cccaaaatgt gtccacagct tcaacagtat gaaatgcatg 2161 gacctgaagg tctacgtgta ggtttttatg agtcagatgt aatgggaaga ggccatgcac 2221 gcctggtgca tgttgaagag cctcacacgg agaccgtacg aaagtacttc cctgagacat 2281 ggatctggga tttggtggtg gtaaactcag caggtgtggc tgaggtagga gtaacagtcc 2341 ctgacaccat caccgagtgg aaggcagggg ccttctgcct gtctgaagat gctggacttg 2401 gtatctcttc cactgcctct ctccgagcct tccagccctt ctttgtggag ctcacaatgc 2461 cttactctgt gattcgtgga gaggccttca cactcaaggc cacggtccta aactaccttc 2521 ccaaatgcat ccgggtcagt gtgcagctgg aagcctctcc cgccttccta gctgtcccag 2581 tggagaagga acaagcgcct cactgcatct gtgcaaacgg gcggcaaact gtgtcctggg 2641 cagtaacccc aaagtcatta ggaaatgtga atttcactgt gagcgcagag gcactagagt 2701 ctcaagagct gtgtgggact gaggtgcctt cagttcctga acacggaagg aaagacacag 2761 tcatcaagcc tctgttggtt gaacctgaag gactagagaa ggaaacaaca ttcaactccc 2821 tactttgtcc atcaggtggt gaggtttctg aagaattatc cctgaaactg ccaccaaatg 2881 tggtagaaga atctgcccga gcttctgtct cagttttggg agacatatta ggctctgcca 2941 tgcaaaacac acaaaatctt ctccagatgc cctatggctg tggagagcag aatatggtcc 3001 tctttgctcc taacatctat gtactggatt atctaaatga aacacagcag cttactccag 3061 aggtcaagtc caaggccatt ggctatctca acactggtta ccagagacag ttgaactaca 3121 aacactatga tggctcctac agcacctttg gggagcgata tggcaggaac cagggcaaca 3181 cctggctcac agcctttgtt ctgaagactt ttgcccaagc tcgagcctac atcttcatcg 3241 atgaagcaca cattacccaa gccctcatat ggctctccca gaggcagaag gacaatggct 3301 gtttcaggag ctctgggtca ctgctcaaca atgccataaa gggaggagta gaagatgaag 3361 tgaccctctc cgcctatatc accatcgccc ttctggagat tcctctcaca gtcactcacc 3421 ctgttgtccg caatgccctg ttttgcctgg agtcagcctg gaagacagca caagaagggg 3481 accatggcag ccatgtatat accaaagcac tgctggccta tgcttttgcc ctggcaggta 3541 accaggacaa gaggaaggaa gtactcaagt cacttaatga ggaagctgtg aagaaagaca 3601 actctgtcca ttgggagcgc cctcagaaac ccaaggcacc agtggggcat ttttacgaac 3661 cccaggctcc ctctgctgag gtggagatga catcctatgt gctcctcgct tatctcacgg 3721 cccagccagc cccaacctcg gaggacctga cctctgcaac caacatcgtg aagtggatca 3781 cgaagcagca gaatgcccag ggcggtttct cctccaccca ggacacagtg gtggctctcc 3841 atgctctgtc caaatatgga gcagccacat ttaccaggac tgggaaggct gcacaggtga 3901 ctatccagtc ttcagggaca ttttccagca aattccaagt ggacaacaac aaccgcctgt 3961 tactgcagca ggtctcattg ccagagctgc ctggggaata cagcatgaaa gtgacaggag 4021 aaggatgtgt ctacctccag acatccttga aatacaatat tctcccagaa aaggaagagt 4081 tcccctttgc tttaggagtg cagactctgc ctcaaacttg tgatgaaccc aaagcccaca 4141 ccagcttcca aaTCTCCCTA AGTGTCAGTT ACACAGGGAG CCGCTCTGCC TCCAACATGG 4201 CGATCGTTGA TGTGAAGATG GTCTCTGGCT TCATTCCCCT GAAGCCAACA GTGAAAATGC 4261 TTGAAAGATC TAACCATGTG AGCCGGACAG AAGTCAGCAG CAACCATGTC TTGATTTACC 4321 TTGATAAGGT GTCAAATCAG ACACTGAGCT TGTTCTTCAC GGTTCTGCAA GATGTCCCAG 4381 TAAGAGATCT GAAACCAGCC ATAGTGAAAG TCTATGATTA CTACGAGACG GATGAGTTTG 4441 CAATTGCTGA GTACAATGCT CCTTGCAGCA AAGATCTTGG AAATGCTTGC CCAACTTTCT 4501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 4561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 4621 GTGGAAAGGA CGAGTCGAGG GACCAGTTCT TAGTAGAACG CGTTAAGTCg acaatcaacc 4681 tctggattac aaaatttgtg aaagatt