Construct: ORF TRCN0000479311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016611.1_s317c1
- Derived from:
- ccsbBroadEn_07861
- DNA Barcode:
- TGCAAACCGATACCCTGAGTACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COBL (23242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_015198.5 | 99.9% | 100% | 213T>C |
| 2 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_017011898.1 | 98.7% | 98.8% | 213T>C;684_728del |
| 3 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515237.1 | 96.8% | 96.9% | 213T>C;684_728del;829_903del |
| 4 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_001346442.2 | 96.2% | 96.2% | 213T>C;3502_3503ins141 |
| 5 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_005271751.1 | 95.6% | 95.5% | 213T>C;1097_1267del |
| 6 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_005271750.1 | 94.5% | 94.5% | 213T>C;684_728del;1142_1312del |
| 7 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515240.1 | 94.3% | 94.4% | 213T>C;784_858del;3577_3578ins141 |
| 8 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515235.1 | 93.8% | 93.8% | 213T>C;784_858del;1172_1342del |
| 9 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515239.1 | 93.2% | 93.3% | (many diffs) |
| 10 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_001346443.2 | 92.8% | 92.7% | (many diffs) |
| 11 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515234.1 | 92.8% | 92.7% | (many diffs) |
| 12 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_001287436.2 | 92% | 92% | 213T>C;1097_1267del;3673_3674ins141 |
| 13 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515236.1 | 89.3% | 89.3% | (many diffs) |
| 14 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_001346441.2 | 88.8% | 88.6% | (many diffs) |
| 15 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515238.1 | 86.2% | 86% | (many diffs) |
| 16 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | XM_011515241.1 | 75.1% | 75% | 0_1ins813;284_454del |
| 17 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_001346444.1 | 34.3% | 29.4% | (many diffs) |
| 18 | human | 23242 | COBL | cordon-bleu WH2 repeat protein | NM_001287438.3 | 29.5% | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3852
- ORF length:
- 3783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgcgccg cgcgcctcgg cggccaagcc cccgaccggg aggaagatga 121 aggctcgtgc tcccccacct cctggaaagg ctgccactct gcatgtgcac agtgaccaga 181 agccccccca cgatggggcc ctcgggtcgc agcagaactt ggttcgcatg aaggaggcgc 241 tgagggccag caccatggac gtcaccgtgg tcctgcctag cgggctggag aagaggagcg 301 tgctcaatgg gagccatgcg atgatggacc tactggttga actttgcctt cagaaccacc 361 tgaatccatc ccaccatgcc cttgaaattc ggtcttcaga aacccaacaa cctttgagtt 421 ttaagccaaa tactttgatt gggaccctga atgtgcatac tgtgtttctg aaagaaaaag 481 ttcctgaaga gaaggttaag cctggtcccc ctaaggtgcc tgagaaatct gtgcgtttgg 541 tcgtgaatta cctgcggaca caaaaagctg ttgtgcgtgt gagccctgag gttcctctcc 601 agaatattct cccagtcatt tgtgcaaagt gtgaggtcag cccagagcac gtggttctcc 661 tcagggacaa cattgccgga gaggagctgg agctgtccaa gtccctgaac gagctcggga 721 taaaggagct ctacgcgtgg gacaacagaa gagaaacctt taggaaatca tcacttggca 781 atgatgagac agataaagag aagaaaaaat ttctgggatt tttcaaagtt aataaaagaa 841 gcaatagtaa gggctgttta acgaccccca actccccatc catgcactca cgttctctta 901 cgctgggtcc atccctctcg ctgggcagca tctcaggggt gtccgtgaag tcggagatga 961 agaagcgccg agcccctcct cctccaggtt cagggccacc tgtgcaagac aaggcatcgg 1021 aaaaggtatc tcttgggtca cagattgatt tacagaagaa gaagcggcga gcgccagctc 1081 cccctccacc acagccacca ccaccgagtc ccctgatccc caaccgcact gaggataagg 1141 aggagaacag gaagagcacg atggtaagcc tgcccctggg gtctggcagc cactgcagcc 1201 cggatggagc cccgcaggtg ctgtcagagg cggaggagac cgtgtcagtt ggcagctgtt 1261 ttgcgtcgga ggacacgacc gaggactcag gagtgatgag ttccccctca gacatcgtct 1321 ctctggactc gcagcaggac agcatgaaat acaaagacaa gtgggccaca gaccaggaag 1381 actgcagtga ccaggacctc gctggaaccc cagacctggg tccccagaag agccccttgt 1441 gggagaagaa tggctctgag aactcacatc tgagaactga aaaagctgtc acagcctcaa 1501 atgatgaaga agacctatta attgctggag agttccgtaa aacccttgca gaacttgatg 1561 aagacctgga agaaatggaa gacagctatg aaacagacac cagctccctc accagctcca 1621 tccatggtgc atccaaccac tgcccacagg atgccatgat ccctcacggc gacacagatg 1681 caatcccagt aacattcata ggggaagttt cagatgatcc tgtggattcg gggttgtttt 1741 ccaatagaaa caacaatgct gggtctttcg actcggaggg tgttgccagc aggagagact 1801 ccctggcgcc acttcaagct gagcacagcc agccccatga aaaggcaagg gaggaagtac 1861 ctgccctgca ccccgcttcc catgacgtcg gaaaaggaat ccgtgtggcc ttatctaaca 1921 tctctaaaga tgggaatcta atggaaacgg cgcccagggt gacttctttt gcttcgaatc 1981 ttcacacaga caacctaaat gcaaaagtga aagacaaagt gtatggctgt gctgacgggg 2041 agaggactca agccacagag agagtgaatt cccaaccggt gaatgaaaag gacagcaacg 2101 ataaaaacgc tgccttggca ccaacatcat ggcaccaacg aggccaaaac ccagggaaaa 2161 gctacagact taagcacggc ctcacaacgt ataaaatcat tcctccaaag tcagagatgc 2221 gatgttacga cagagatgtg tccctctcca ccggagccat taagattgac gagctgggga 2281 acttggtgag tcctcacgcc accggcatca ggatcatttc cctgtcttcg tctgtgcctg 2341 aagcagaatc tcagcccatt gggaaagtca gagagttctg gaggtgcaac tctgtggaaa 2401 aacacctggg ccgaccctca gagagctctg ccaggggacc cccctccacc cctgtgccca 2461 cgcagacgca gaatccagag agcagactcc aagcagaccc caagccaata tcgccccagc 2521 agaagtctgc ccaccatgag ggccggaacc ccctagggga ggggagaaac cagcccccca 2581 ccatgggcat gggtcacgtg agggtgccag cagctcacac cacagaagtc acatttctca 2641 agcctcagag aagaacgtcc agccagtatg tggcctctgc cattgccaag cgcataggag 2701 ccccaaaagt ccatgctgat gtggtgaggc cacacggtta tgccgagaag ggttatgcag 2761 gcaaggcgcc agtgctggct gcaccacctg tcactgtgaa agatgacagg acatccagcc 2821 cacactcaga gacacaagga tggaaggatg gtgcccagtg gccctgtgtc actcctccca 2881 acaaccacgg ggaagatttg gcagtgggag cccctcctag gggggaggtc attggcccac 2941 acaggaagtt gtctactcag gacagacctg ctgctatcca cagaagctcc tgtttctcac 3001 tggttcagtc ttcccagagg gatcgtgttt ctgtgggaca gagctgtggt ttcagtggaa 3061 agcaaagcac aagtagccag gaggccagct cagcatctga gcccagacgc gcacctgatg 3121 gtacagaccc accccctcca cacacatctg acactcaggc ctgcagcagg gagctggtca 3181 atggctctgt gcgcgcccca ggccacggcg agccttccca ccctccagga gggtctggca 3241 cagaaagcca cattctccta gaaagagagg aaaagcccag tgtgttctct acagatggaa 3301 atgaaacaga cagtatttgg ccacccagca tttttgggcc gaagaaaaaa ttcaaacctg 3361 ttgtccagag accagtccca aaagacacat ccctgcactc tgccctgatg gaagccatcc 3421 actctgcggg agggaaggac agactacgca agacggcaga acacaccggg gagggcaggc 3481 cagcgaaact gtcctacacg gaggcagagg gcgaacgatc tgcactgctg gcagctatcc 3541 gcgggcacag cggcacctgc agcctgagga aggtggcatc ctctgcttct gaggagctcc 3601 agagcttccg agatgccgca ctctctgctc agggctcgga aagtcctctg ctagaagacc 3661 ttggtcttct gtccccacca gcCATTCCCC CACCACCCCC TCCACCCTCC CAGGCTCTCT 3721 CTGCACCAAG GACGGCCTCC AGGTTCAGCA CGGGCACCCT CAGCAACACC GCAGACGCAA 3781 GGCAAGCCTT GATGGACGCC ATCCGCTCCG GCACAGGGGC TGCGAGACTG AGAAAGGTGC 3841 CCTTGCTCGT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC AAACCGATAC CCTGAGTACC 4021 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t