Construct: ORF TRCN0000479312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006440.1_s317c1
- Derived from:
- ccsbBroadEn_04814
- DNA Barcode:
- CTTACCCAAAAACGGGGCAACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MISP (126353)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126353 | MISP | mitotic spindle positioning | NM_173481.4 | 100% | 100% | |
| 2 | human | 126353 | MISP | mitotic spindle positioning | XM_011527685.2 | 100% | 100% | |
| 3 | human | 126353 | MISP | mitotic spindle positioning | XM_011527686.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2103
- ORF length:
- 2037
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccgcgtgacc agatacccca tcctgggcat ccctcaggca caccgtggca 121 ccggcctggt gctggatgga gacaccagct acacatacca tctggtgtgc atgggccccg 181 aggccagcgg ctggggccag gatgagccgc agacatggcc cactgaccac agggcccagc 241 agggcgtgca gaggcagggg gtgtcctaca gcgtgcatgc ctacactggc cagccgtccc 301 cacgggggct ccactcggag aacagggagg atgagggttg gcaggtttac cgcctgggcg 361 ccagggatgc ccaccaggga cgtccaacat gggcactccg cccagaggac ggggaggaca 421 aggagatgaa gacctaccgc ctggatgctg gggacgctga ccccaggagg ctgtgtgacc 481 tggagcggga gcgctgggcc gtcatccagg gccaggcagt caggaagagc agcaccgtgg 541 ccacgctcca gggcactcct gaccacggag accccaggac ccccggccca cctcggtcca 601 cgcccctgga ggagaacgtg gttgacaggg agcagattga cttcctggca gcgagacagc 661 agttcctgag tctggagcag gcgaacaagg gggcccctca tagctccccg gccaggggga 721 cccctgcagg cacaacccca ggggccagcc aggcccccaa ggccttcaac aagccccacc 781 tggccaacgg gcacgtggtt cccatcaagc cccaggtgaa gggggtggtc agggaagaga 841 acaaggtgcg tgctgtgccc acctgggcca gtgtccaagt tgtggatgac cctggctcct 901 tggcctcagt ggagtccccg gggaccccca aggagacgcc catcgagcgg gagatccgtc 961 tggctcagga gcgtgaggca gacctgcgag agcagagggg gcttcggcag gcaaccgacc 1021 accaggagct ggtggaaatc cccaccaggc cgctgctgac caagctgagc ctgatcacag 1081 ccccacggcg ggagagaggg cgcccgtccc tctacgtgca gcgggacata gtacaggaga 1141 cacagcgtga ggaagaccac cggcgggagg gcctgcacgt gggccgggcg tccacacccg 1201 actgggtctc ggagggtccc cagcccggac tccggagagc cctcagctca gattccatcc 1261 tcagcccggc cccagatgcc cgtgcggccg acccagctcc agaagtgagg aaggtgaacc 1321 gcatcccacc tgatgcctac cagccgtacc tgagccccgg gaccccccag ctagaattct 1381 cagccttcgg agcattcggc aagcccagca gtctctccac agcggaggcc aaggctgcga 1441 cttcaccaaa ggccacgatg tccccgaggc atctctcaga atcctctgga aaacccctga 1501 gcacaaagca agaggcatcg aagccccctc ggggatgccc gcaagccaac aggggtgtcg 1561 tgcggtggga gtacttccgc ctgcgtcctc tgcggttcag ggccccagac gagccccagc 1621 aggcccaagt cccccatgtc tggggctggg aggtggctgg ggcccctgca ctgaggctgc 1681 agaagtccca gtcatctgat ctgctggaaa gggagaggga gagtgtcctg cgccgggagc 1741 aagaggtggc agaggagcgg agaaatgctc tcTTCCCAGA GGTCTTCTCC CCAACGCCAG 1801 ATGAGAACTC TGACCAGAAC TCCAGGAGCT CCTCCCAGGC ATCCGGCATC ACGGGCAGTT 1861 ACTCGGTGTC TGAGTCTCCC TTCTTCAGCC CCATCCACCT ACACTCAAAC GTGGCGTGGA 1921 CAGTGGAAGA TCCAGTGGAC AGTGCTCCTC CCGGGCAGAG AAAGAAGGAG CAATGGTACG 1981 CTGGCATCAA CCCCTCGGAC GGTATCAACT CAGAGGTCCT GGAAGCCATA CGGGTGACCC 2041 GTCACAAGAA CGCCATGGCA GAGCGCTGGG AATCCCGCAT CTACGCCAGT GAGGAGGATG 2101 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2161 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2221 GGCTTTATAT ATCTTGTGGA AAGGACGACT TACCCAAAAA CGGGGCAACC CCACGCGTTA 2281 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt