Construct: ORF TRCN0000479318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003399.1_s317c1
- Derived from:
- ccsbBroadEn_02659
- DNA Barcode:
- GTCATTAGCAAAACTACGGTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A14 (11254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11254 | SLC6A14 | solute carrier family 6 mem... | NM_007231.5 | 100% | 100% | |
| 2 | mouse | 56774 | Slc6a14 | solute carrier family 6 (ne... | NM_020049.4 | 87.6% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1995
- ORF length:
- 1926
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacaagttg aaatgcccga gtttcttcaa gtgcagggag aaggagaaag 121 tgtcggcttc atcagagaat ttccatgttg gtgaaaatga tgagaatcag gaccgtggta 181 actggtccaa aaaatcggat tatcttctat ctatgattgg atacgcagtg ggattaggaa 241 atgtgtggag atttccatat ctgacctaca gcaatggtgg aggcgccttc ttgatacctt 301 atgcaattat gttagcattg gctggtttac ctttgttctt tctggagtgt tcactgggac 361 aatttgctag cttaggtcca gtttcagttt ggaggattct tccattgttt caaggtgtgg 421 gaattacaat ggtcctgatc tccatttttg tgacaatcta ttacaatgtc ataattgcct 481 atagtcttta ctacatgttt gcttcttttc aaagtgaact accatggaaa aattgttctt 541 cgtggtcaga taaaaactgt agcagatcac caatagtaac tcactgtaat gtgagtacag 601 tgaataaagg aatacaagag atcatccaaa tgaataaaag ctgggtagac atcaacaatt 661 ttacctgcat caacggcagt gaaatttatc agccagggca gcttcccagt gaacaatatt 721 ggaataaagt ggcgctccaa cggtcaagtg gaatgaatga gactggagta attgtttggt 781 atttagcact ttgtcttctt ctggcttggc tcatagttgg agcagcacta tttaaaggaa 841 tcaaatcgtc tggcaaggtg gtatatttta cagctctttt cccctatgtg gtcctactca 901 tcctgttagt acgaggtgca actctggagg gtgcttcaaa aggcatttca tactatattg 961 gagcccagtc aaattttaca aaacttaagg aagctgaggt atggaaagat gctgccactc 1021 agatatttta ctccctttca gtggcttggg gtggcttagt tgctctatca tcttacaata 1081 agttcaaaaa caactgcttc tctgatgcca ttgtggtttg tttgacaaac tgtctcacta 1141 gcgtgtttgc tggatttgct attttttcta tattgggaca catggcccat atatctggaa 1201 aggaagtttc tcaagttgta aaatcaggtt ttgatttggc attcattgcc tatccagagg 1261 ctctagccca actcccaggt ggtccatttt ggtccatatt attttttttc atgcttttaa 1321 ctttgggtct cgattctcag tttgcttcga ttgaaacgat cacaacaaca attcaagatt 1381 tatttcccaa agtgatgaag aaaatgaggg ttcccataac tttgggctgc tgcttggttt 1441 tgtttctcct tggtctcgtc tgtgtgactc aggctggaat ttactgggtt catctgattg 1501 accacttctg tgctggatgg ggcattttaa ttgcagctat actggagcta gttGGAATCA 1561 TCTGGATTTA TGGAGGGAAC AGATTCATTG AGGATACAGA AATGATGATT GGAGCAAAGA 1621 GGTGGATATT CTGGCTATGG TGGAGAGCTT GCTGGTTTGT AATTACGCCT ATCCTTTTGA 1681 TTGCAATATT TATCTGGTCA TTGGTGCAAT TTCATAGACC TAATTATGGC GCAATTCCAT 1741 ACCCTGACTG GGGAGTTGCT TTAGGCTGGT GTATGATTGT TTTCTGCATT ATTTGGATTC 1801 CAATTATGGC TATCATAAAA ATAATTCAGG CTAAAGGAAA CATCTTTCAA CGCCTTATAA 1861 GTTGCTGCAG ACCAGCTTCT AACTGGGGTC CATACCTGGA ACAACATCGT GGGGAAAGAT 1921 ATAAAGACAT GGTAGATCCT AAAAAAGAGG CTGACCATGA AATACCTACT GTTAGTGGCA 1981 GCAGAAAACC GGAATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2041 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2101 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTCATTAGCA AAACTACGGT 2161 AACCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt