Construct: ORF TRCN0000479325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001355.1_s317c1
- Derived from:
- ccsbBroadEn_12326
- DNA Barcode:
- TTCCAGAAATAACCTAGAAACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASS4 (57091)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57091 | CASS4 | Cas scaffold protein family... | NM_001164116.2 | 84.7% | 82.4% | (many diffs) |
| 2 | human | 57091 | CASS4 | Cas scaffold protein family... | NM_020356.4 | 84.7% | 82.4% | (many diffs) |
| 3 | human | 57091 | CASS4 | Cas scaffold protein family... | NM_001164114.2 | 77.8% | 75.6% | (many diffs) |
| 4 | human | 57091 | CASS4 | Cas scaffold protein family... | XM_006723831.3 | 77.8% | 75.6% | (many diffs) |
| 5 | human | 57091 | CASS4 | Cas scaffold protein family... | NM_001164115.2 | 45.2% | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2082
- ORF length:
- 2016
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gggaacaggc atcatggact gtgcgcccaa ggcactcctg gccagggcac 121 tttatgacaa ctgccctgac tgctctgacg agctggcttt cagcagaggg gacatcctga 181 ccattctgga gcaacacgtg ccagaaagcg agggttggtg gaagtgtttg ctccatggga 241 ggcaaggcct ggcccctgcc aaccgcctcc aaatcctcac ggaggtcgct gcagacaggc 301 cgtgcccccc attcctgaga ggcctggaag aagctcctgc cagctcagag gagacctatc 361 aggtgcccac tctaccccgc cctcccactc caggccccgt ttatgagcag atgaggagtt 421 gggcggaggg gccccagccc cctactgccc aagtctatga attccccgac cctcccacca 481 gtgccagaat catctgtgaa aagactctca gctttccaaa acaggccatc ctcacgcttc 541 ccagacctgt ccgggcctca ctgccgactc tgccttccca ggtgtatgac gtgcctaccc 601 agcaccgggg ccccgtggtc ctgaaggagc cagagaagca gcagttatat gacataccag 661 ccagccccaa gaaggcagga ctccatcccc cagacagcca agcaagtggg cagggtgttc 721 ccctgatatc agtgactacc ttaagaagag gcggttacag cacattacca aatcctcaga 781 aatcggaatg gatttatgac actccagtgt ctccaggaaa ggccagcgtc agaaacacgc 841 ctctcaccag ctttgcggaa gaatcaaggc cccacgctct ccccagttcc agctccactt 901 tctacaatcc tccaagtggc agatccaggt ccctcactcc acaactgaat aacaatgtgc 961 ccatgcagaa aaaactcagc cttccagaaa ttccttctta tggctttctt gtacccagag 1021 gcacatttcc tttggatgaa gatgtcagct acaaggttcc ttcaagcttt ctgattcccc 1081 gagtggaaca gcagaacacc aagcccaata tttatgacat ccctaaagca acgtcgagtg 1141 tttctcaggc tgggaaggag ctggagaaag ccaaggaggt gtcagagaat tccgcgggcc 1201 ataattcctc atggttctcc agacggacaa cttccccatc tcctgaaccg gacagattat 1261 caggttccag ttctgacagc agagctagca tcgtttcctc gtgctccacc acatccaccg 1321 acgactcctc cagctcttcc tcggaggagt cagcaaagga gctctccttg gacctggatg 1381 tggccaagga gacagtgatg gctctgcagc acaaggtggt cagctctgtc gctggcctga 1441 tgctctttgt cagcaggaag tggagattcc gagactatct ggaggccaac attgatgcaa 1501 tccacaggtc cactgatcac atagaagaat ctgtaagaga atttctggat tttgcccgag 1561 gagtccatgg gactgcctgt aacctcactg acagtaacct tcagaacaga attcgggacc 1621 agatgcagac catctccaac tcctaccgca tcctgcttGA AACAAAGGAA AGCTTGGATA 1681 ATCGCAATTG GCCTCTGGAA GTTCTTGTGA CTGACAGTGT CCAGAACAGC CCAGATGACC 1741 TTGAGAGGTT TGTCATGGTG GCACGGATGC TTCCAGAAGA CATCAAGAGG TTTGCCTCCA 1801 TTGTCATTGC CAATGGAAGG CTCCTTTTTA AGCGGAACTG TGAAAAGGAA GAGACTGTGC 1861 AGTTGACCCC AAATGCAGAA TTTAAGTGTG AAAAATACAT CCAGCCTCCC CAAAGAGAAA 1921 CTGAATCACA CCAAAAGAGT ACCCCTTCCA CTAAGCAAAG GGAAGATGAA CACTCTTCTG 1981 AACTATTAAA GAAAAATAGG GCAAATATCT GTGGACAGGT GAGTTCAGAG TTCCAAGTGA 2041 TCGAAAAAGG GGCTTCAATT GTTACCTGGA GTAGTGGCTA CTACCCAACT TTCTTGTACA 2101 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2161 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2221 AGGACGATTC CAGAAATAAC CTAGAAACCG CACGCGTTAA GTCgacaatc aacctctgga 2281 ttacaaaatt tgtgaaagat t