Construct: ORF TRCN0000479335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002123.1_s317c1
- Derived from:
- ccsbBroadEn_09291
- DNA Barcode:
- GTTTTGCCACCTTTAACAGCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA2013 (90231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479335
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90231 | KIAA2013 | KIAA2013 | NM_138346.3 | 99.9% | 100% | 297C>A |
| 2 | mouse | 77034 | 2510039O18Rik | RIKEN cDNA 2510039O18 gene | NM_029841.3 | 87.9% | 91.9% | (many diffs) |
| 3 | mouse | 77034 | 2510039O18Rik | RIKEN cDNA 2510039O18 gene | XM_006539279.3 | 80.5% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1968
- ORF length:
- 1902
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gctgcagcag cggctcaagg ggctgccggg actgctgtcg agcagctggg 121 cccgccgcct cctctgcctg cttggcctcc tgctgctgct tctgtggttt ggggggtccg 181 gcgcgcggcg ggcggcgggc ggcctgcacc tgctgccctg gtcccgcggt gagccgggcg 241 ccgccgagcc gtctgcctgc ctggaggcgg ccacccgcgc ctggcgcggc ctgcgggagc 301 gcggtgaggt ggtaccgctg ggtcctggag tgccggccct ggtggccaac ggcttcctgg 361 cactggacgt ggctgccaat cggctgtggg tgactcccgg ggagcgggag cccgccgtgg 421 cgccggactt tgtgcccttc gtgcagctgc gcccgctgag cgcgctggct gaagctggag 481 aggcggtgct gctgctgcgg gaggggcttc tgcgccgcgt gcgttgcctg cagctggggt 541 ccccaggtcc tggccccgtg gccgccggcc ccgggcccgc ctccgtctct ggccttgccg 601 cggggtccgg ccgcgactgc gtgctgctgc aagaggactt tctggcgcac aggggccgac 661 cccacgtcta cctgcagcgc atccagctca acaaccccac ggagcgcgtg gccgcgctgc 721 agactgtggg gcccactgcc ggcccagccc ccaaggcctt caccagtacc ctggagaagg 781 tcggagacca tcagttcctc ctctactcag gccggtcccc gcctacgccc actgggttgg 841 tgcacctggt ggtggtggcc gccaagaagc tggtgaaccg cctccaagtg gctcccaaga 901 cgcagctgga tgagacggtg ctgtgggtgg tgcacgtctc tggccccatt aacccccagg 961 tgctcaaaag caaagcagcc aaggagctca aggcgctgca ggacttggca cggaaggaaa 1021 tgctggagct cttggacatg ccagcggcgg agctgcttca agaccaccag ctcctctggg 1081 ctcagctctt cagcccagga gtggaaatga agaagatcac tgacacccac acgccgtctg 1141 gcctcaccgt gaacctgacg ctctattaca tgctctcctg ctcgccagcc ccactgctca 1201 gcccctccct gagccacagg gagcgagacc agatggagtc gacgctcaac tatgaagatc 1261 actgcttcag cgggcacgcc accatgcacg ccgagaacct gtggccgggg cggctgtcct 1321 ccgtccagca gatcctgcag ctctctgacc tgtggaggct gaccctccag aagcgtggct 1381 gcaaggggct ggtgaaggtg ggtgccccag gcatcctgca ggggatggtg ctcagctttg 1441 gggggctgca gttcacagag aaccacctcc agttccaggc cgaccccGAC GTGCTGCACA 1501 ACAGCTATGC ATTGCATGGC ATCCGCTACA AGAACGACCA TATCAACCTG GCCGTGCTGG 1561 CGGATGCCGA GGGCAAGCCC TACCTACACG TGTCCGTGGA GTCCCGTGGC CAGCCTGTCA 1621 AGATCTATGC CTGCAAGGCA GGCTGCCTGG ACGAGCCAGT GGAGCTGACC TCGGCGCCCA 1681 CGGGCCACAC CTTCTCGGTC ATGGTGACAC AGCCCATCAC GCCACTGCTC TACATCTCCA 1741 CCGACCTCAC ACACCTGCAG GACCTGCGGC ACACGCTGCA CCTCAAGGCC ATCCTGGCCC 1801 ATGATGAGCA CATGGCCCAG CAGGACCCCG GGCTGCCCTT CCTCTTCTGG TTCAGCGTGG 1861 CCTCCCTAAT CACCCTCTTC CACCTCTTCC TCTTCAAGCT CATCTACAAC GAGTACTGTG 1921 GGCCTGGAGC CAAGCCCCTC TTCAGGAGTA AGGAAGATCC CAGTGTCTGC CCAACTTTCT 1981 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2041 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2101 GTGGAAAGGA CGAGTTTTGC CACCTTTAAC AGCCTTTACG CGTTAAGTCg acaatcaacc 2161 tctggattac aaaatttgtg aaagatt