Construct: ORF TRCN0000479345
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008347.1_s317c1
- Derived from:
- ccsbBroadEn_12045
- DNA Barcode:
- GGCCCGGCTTGTCCAAAAACACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHPF2 (54480)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479345
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54480 | CHPF2 | chondroitin polymerizing fa... | NM_019015.2 | 84.9% | 83.2% | (many diffs) |
| 2 | human | 54480 | CHPF2 | chondroitin polymerizing fa... | NM_001284295.2 | 83.2% | 81% | (many diffs) |
| 3 | mouse | 100910 | Chpf2 | chondroitin polymerizing fa... | NM_133913.2 | 76.3% | 79.8% | (many diffs) |
| 4 | mouse | 100910 | Chpf2 | chondroitin polymerizing fa... | XM_017320583.1 | 76.3% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2064
- ORF length:
- 1998
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg actgagctcc ctgttggctc tgctgcggcc agcgcttccc ctcatcttag 121 ggctgtctct ggggtgcagc ctgagcctcc tgcgggtttc ctggatccag ggggagggag 181 aagatccctg tgtcgaggct gtaggggagc gaggagggcc acagaatcca gattcgagag 241 ctcggctaga ccaaagtgat gaagacttca aaccccggat tgtcccctac tacagggacc 301 ccaacaagcc ctacaagaag gtgctcagga ctcggtacat ccagacagag ctgggctccc 361 gtgagcggtt gctggtggct gtcctgacct cccgagctac actgtccact ttggccgtgg 421 ctgtgaaccg tacggtggcc catcacttcc ctcggttact ctacttcact gggcagcggg 481 gggcccgggc tccagcaggg atgcaggtgg tgtctcatgg ggatgagcgg cccgcctggc 541 tcatgtcaga gaccctgcgc caccttcaca cacactttgg ggccgactac gactggttct 601 tcatcatgca ggatgacaca tatgtgcagg ccccccgcct ggcagccctt gctggccacc 661 tcagcatcaa ccaagacctg tacttaggcc gggcagagga gttcattggc gcaggcgagc 721 aggcccggta ctgtcatggg ggctttggct acctgttgtc acggagtctc ctgcttcgtc 781 tgcggccaca tctggatggc tgccgaggag acattctcag tgcccgtcct gacgagtggc 841 ttggacgctg cctcattgac tctctgggcg tcggctgtgt ctcacagcac caggggcagc 901 agtatcgctc atttgaactg gccaaaaata gggaccctga gaaggaaggg agctcggctt 961 tcctgagtgc cttcgccgtg caccctgtct ccgaaggtac cctcatgtac cggctccaca 1021 aacgcttcag cgctctggag ttggagcggg cttacagtga aatagaacaa ctgcaggctc 1081 agatccggaa cctgaccgtg ctgacccccg aaggggaggc agggctgagc tggcccgttg 1141 ggctccctgc tcctttcaca ccacactctc gctttgaggt gctgggctgg gactacttca 1201 cagagcagca caccttctcc tgtgcagatg gggctcccaa gtgcccacta cagggggcta 1261 gcagggcgga cgtgggtgat gcgttggaga ctgccctgga gcagctcaat cggcgctatc 1321 agccccgcct gcgcttccag aagcagcgac tgctcaacgg ctatcggcgc ttcgacccag 1381 cacggggcat ggagtacacc ctggacctgc tgttggaatg tgtgacacag cgtgggcacc 1441 ggcgggccct ggctcgcagg gtcagcctgc tgcggccact gagccgggtg gaaatcctac 1501 ctatgcccta tgtcactgag gccacccgag tgcagctggt gctgccactc ctggtggctg 1561 aagctgctgc agccccggct ttcctcgagg cgtttgcagc caatgtcctg gagccacgag 1621 aacatgcatt gctcaccctg ttgctggtcT ACGGGCCACG AGAAGGTGGC CGTGGAGCTC 1681 CAGACCCATT TCTTGGGGTG AAGGCTGCAG CAGCGGAGTT AGAGCGACGG TACCCTGGGA 1741 CGAGGCTGGC CTGGCTCGCT GTGCGAGCAG AGGCCCCTTC CCAGGTGCGA CTCATGGACG 1801 TGGTCTCGAA GAAGCACCCT GTGGACACTC TCTTCTTCCT TACCACCGTG TGGACAAGGC 1861 CTGGGCCCGA AGTCCTCAAC CGCTGTCGCA TGAATGCCAT CTCTGGCTGG CAGGCCTTCT 1921 TTCCAGTCCA TTTCCAGGAG TTCAATCCTG CCCTGTCACC ACAGAGATCA CCCCCTAACC 1981 TCATTACCTT TCCTTTGTCT GCCTCAGCCC CAGGAAGGGC AAGGCAAGAT GGTGGACAGA 2041 TAGAGAATTG TTGCTGTATT TTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GCCCGGCTTG 2221 TCCAAAAACA CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att