Construct: ORF TRCN0000479349

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009573.1_s317c1
Derived from:
ccsbBroadEn_06019
DNA Barcode:
CACACTCTCAAGGACTCGCCTTGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COPA (1314)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479349
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1314 COPA COPI coat complex subunit a... NM_001098398.2 99.9% 100% 363C>T
2 human 1314 COPA COPI coat complex subunit a... NM_004371.4 99.2% 99.2% 363C>T;1527_1528ins27
3 mouse 12847 Copa coatomer protein complex su... NM_009938.4 90.3% 97.7% (many diffs)
4 mouse 12847 Copa coatomer protein complex su... XM_011238748.2 64.5% 68.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3765
ORF length:
3699
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttgacatgtt aaccaaattc gagaccaaga gcgcgcgggt caaagggctc agctttcacc
121ccaaaagacc ttggatcctg actagtttac ataatggggt catccagtta tgggactatc
181ggatgtgcac tctcattgac aagtttgatg aacatgatgg tccagtgcga ggcattgact
241tccataagca gcagccactg ttcgtctctg gaggagatga ctataagatt aaggtttgga
301attacaagct tcggcgctgt cttttcacat tgcttgggca cttagattat attcgcacca
361cgttttttca tcatgaatat ccctggattc tgagtgcctc cgatgatcag accatccgag
421tgtggaattg gcaatctaga acctgtgttt gtgtgttaac agggcacaac cattatgtga
481tgtgtgctca gttccacccc acagaagact tggtagtatc agccagcctg gaccagactg
541tgcgcgtttg ggatatttct ggtctgagga aaaaaaacct gtcccctggt gcggtggaat
601cggatgtgag aggaataact ggggttgatc tatttggaac tacagatgca gtggtgaagc
661atgtactaga gggtcacgat cgtggagtaa actgggctgc cttccacccc actatgcccc
721ttattgtatc tggggcagat gatcgtcaag tgaagatctg gcgcatgaat gaatcaaagg
781catgggaggt tgatacctgc cggggccatt acaacaatgt atcttgtgcc gtcttccacc
841ctcgccaaga gttgatcctc agcaattctg aggacaagag tattcgagtc tgggatatgt
901ctaagcggac tggggttcag actttccgca gagaccatga tcgtttctgg gtcctagctg
961ctcaccctaa ccttaacctc tttgcagcag gccatgatgg tggtatgatt gtgtttaagc
1021tggaacggga acggccagcc tatgctgttc atggcaatat gctacactat gtcaaggacc
1081gattcttacg acagctggat ttcaacagct ccaaagatgt agctgtgatg cagttgcgga
1141gtggttccaa gtttccagta ttcaatatgt catacaatcc agcagaaaat gcagtcctgc
1201tttgtacaag agctagcaat ctagagaata gtacctatga cctgtacacc atccctaaag
1261atgctgactc ccagaatcct gatgcgcctg aagggaaacg atcctcaggc ctgacagccg
1321tttgggtcgc tcgaaatcgg tttgctgtcc tagatcggat gcattcgctt ctgatcaaga
1381atctgaagaa tgagatcacc aaaaaggtac aggtgcccaa ctgtgatgag atcttctatg
1441ctggcacagg caatctcctg cttcgagatg cggactctat cacactcttt gacgtacagc
1501agaagcggac tctggcatct gtgaagattt ctaaagtgaa atacgttatc tggtcagcag
1561acatgtcaca tgtagcacta ctagccaaac acgaacactc atgccctttg cctcttacag
1621ccattgtgat ctgtaaccgc aaactggatg ctttatgtaa cattcatgag aacattcgtg
1681tcaagagtgg ggcctgggat gagagtgggg tatttatcta taccacaagc aaccacatca
1741aatatgctgt caccactggg gaccacggga tcattcgaac tctggattta cccatctatg
1801tcacacgggt gaagggcaac aatgtatact gcctagacag ggagtgtcgt ccccgggtac
1861tcaccattga tcccactgag ttcaaattca agctggccct gatcaacaga aaatatgatg
1921aggtactgca catggtgagg aatgccaaac tagttggcca gtctattatt gcttatctcc
1981agaagaaggg ctatcctgaa gtggcactgc attttgtcaa ggatgagaaa actcgcttta
2041gtctggcact ggagtgtgga aacattgaga ttgctctgga agcagccaaa gcactggatg
2101acaagaactg ctgggaaaag ctgggagaag tggccctgct gcaggggaac caccagattg
2161tggaaatgtg ctatcagcgt accaaaaact ttgacaaact ttccttcctg tatcttatca
2221ctggcaactt agaaaaactt cgcaagatga tgaagattgc tgagatcaga aaggacatga
2281gtggccacta tcagaatgcc ctatacctgg gtgatgtgtc agagcgtgtg cggatcctga
2341agaactgtgg acagaagtcc ctggcctatc tcacagctgc tacccatggc ttagatgaag
2401aagctgagag cctaaaggag acatttgacc cagagaagga gacaatccca gacattgacc
2461ctaatgccaa gctgctccag ccacctgcac ctatcatgcc attggatacc aattggcctt
2521tattgactgt atccaaagga ttttttgaag gcaccattgc cagcaaaggg aagggaggag
2581cactggctgc tgacattgac attgacactg ttggtacaga gggctgggga gaggatgcag
2641agctgcagtt ggatgaagat gggtttgtgg aggctacaga aggtttgggg gatgatgctc
2701ttggcaaggg acaggaagaa ggaggtggct gggatgtaga agaagatctg gagctccctc
2761ctgagctgga tatatcccct ggggcagctg gtggggctga agatggtttc tttgtgcccc
2821caaccaaggg aacaagtcca actcagatct ggtgtaataa ctctcagctt ccagttgatc
2881acatcctggc aggctctttc gaaacagcca tgcggctcct tcatgaccaa gtaggggtaa
2941tccagtttgg cccctacaag caactgttcc tacagacata cgcccgaggc cgcacaacct
3001atcaggctct gccctgccta ccctccatgt atggctatcc taatcgcaac tggaaggatg
3061cagggctgaa gaatggtgta ccagctgtgg gcctgaagct taatgacctc atccaacggt
3121tgcagctgtg ctaccagctc accacagttg gcaaatttga ggaggctgtg gaaaaattcc
3181gttccatcct tctcagtgtg ccacttcttg ttgtggacaa taaacaagag atTGCAGAGG
3241CCCAGCAGCT CATCACCATT TGCCGTGAGT ACATTGTGGG TTTGTCCGTG GAGACAGAAA
3301GGAAGAAGCT GCCCAAAGAG ACTCTAGAAC AGCAGAAGCG CATCTGTGAG ATGGCAGCCT
3361ATTTCACCCA CTCAAACCTG CAGCCTGTGC ACATGATCCT GGTGCTGCGT ACAGCCCTCA
3421ATCTGTTCTT CAAGCTCAAG AACTTCAAGA CAGCTGCCAC CTTTGCTCGG CGCCTACTAG
3481AACTCGGGCC CAAGCCTGAG GTGGCCCAAC AGACCCGAAA AATCCTGTCT GCCTGTGAGA
3541AGAATCCCAC AGATGCCTAC CAGCTCAATT ATGACATGCA CAACCCCTTT GACATTTGTG
3601CTGCATCATA TCGGCCCATC TACCGTGGAA AGCCAGTAGA AAAGTGTCCA CTCAGTGGGG
3661CCTGCTATTC CCCTGAGTTC AAAGGTCAAA TCTGCAGGGT CACCACAGTG ACAGAGATTG
3721GCAAAGATGT GATTGGTTTA AGGATCAGTC CTCTGCAGTT TCGCTACCAC TTTCTTGTAC
3781AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG
3841TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA
3901AAGGACGACA CACTCTCAAG GACTCGCCTT GGACGCGTTA AGTCgacaat caacctctgg
3961attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)