Construct: ORF TRCN0000479349
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009573.1_s317c1
- Derived from:
- ccsbBroadEn_06019
- DNA Barcode:
- CACACTCTCAAGGACTCGCCTTGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COPA (1314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479349
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1314 | COPA | COPI coat complex subunit a... | NM_001098398.2 | 99.9% | 100% | 363C>T |
| 2 | human | 1314 | COPA | COPI coat complex subunit a... | NM_004371.4 | 99.2% | 99.2% | 363C>T;1527_1528ins27 |
| 3 | mouse | 12847 | Copa | coatomer protein complex su... | NM_009938.4 | 90.3% | 97.7% | (many diffs) |
| 4 | mouse | 12847 | Copa | coatomer protein complex su... | XM_011238748.2 | 64.5% | 68.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3765
- ORF length:
- 3699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgacatgtt aaccaaattc gagaccaaga gcgcgcgggt caaagggctc agctttcacc 121 ccaaaagacc ttggatcctg actagtttac ataatggggt catccagtta tgggactatc 181 ggatgtgcac tctcattgac aagtttgatg aacatgatgg tccagtgcga ggcattgact 241 tccataagca gcagccactg ttcgtctctg gaggagatga ctataagatt aaggtttgga 301 attacaagct tcggcgctgt cttttcacat tgcttgggca cttagattat attcgcacca 361 cgttttttca tcatgaatat ccctggattc tgagtgcctc cgatgatcag accatccgag 421 tgtggaattg gcaatctaga acctgtgttt gtgtgttaac agggcacaac cattatgtga 481 tgtgtgctca gttccacccc acagaagact tggtagtatc agccagcctg gaccagactg 541 tgcgcgtttg ggatatttct ggtctgagga aaaaaaacct gtcccctggt gcggtggaat 601 cggatgtgag aggaataact ggggttgatc tatttggaac tacagatgca gtggtgaagc 661 atgtactaga gggtcacgat cgtggagtaa actgggctgc cttccacccc actatgcccc 721 ttattgtatc tggggcagat gatcgtcaag tgaagatctg gcgcatgaat gaatcaaagg 781 catgggaggt tgatacctgc cggggccatt acaacaatgt atcttgtgcc gtcttccacc 841 ctcgccaaga gttgatcctc agcaattctg aggacaagag tattcgagtc tgggatatgt 901 ctaagcggac tggggttcag actttccgca gagaccatga tcgtttctgg gtcctagctg 961 ctcaccctaa ccttaacctc tttgcagcag gccatgatgg tggtatgatt gtgtttaagc 1021 tggaacggga acggccagcc tatgctgttc atggcaatat gctacactat gtcaaggacc 1081 gattcttacg acagctggat ttcaacagct ccaaagatgt agctgtgatg cagttgcgga 1141 gtggttccaa gtttccagta ttcaatatgt catacaatcc agcagaaaat gcagtcctgc 1201 tttgtacaag agctagcaat ctagagaata gtacctatga cctgtacacc atccctaaag 1261 atgctgactc ccagaatcct gatgcgcctg aagggaaacg atcctcaggc ctgacagccg 1321 tttgggtcgc tcgaaatcgg tttgctgtcc tagatcggat gcattcgctt ctgatcaaga 1381 atctgaagaa tgagatcacc aaaaaggtac aggtgcccaa ctgtgatgag atcttctatg 1441 ctggcacagg caatctcctg cttcgagatg cggactctat cacactcttt gacgtacagc 1501 agaagcggac tctggcatct gtgaagattt ctaaagtgaa atacgttatc tggtcagcag 1561 acatgtcaca tgtagcacta ctagccaaac acgaacactc atgccctttg cctcttacag 1621 ccattgtgat ctgtaaccgc aaactggatg ctttatgtaa cattcatgag aacattcgtg 1681 tcaagagtgg ggcctgggat gagagtgggg tatttatcta taccacaagc aaccacatca 1741 aatatgctgt caccactggg gaccacggga tcattcgaac tctggattta cccatctatg 1801 tcacacgggt gaagggcaac aatgtatact gcctagacag ggagtgtcgt ccccgggtac 1861 tcaccattga tcccactgag ttcaaattca agctggccct gatcaacaga aaatatgatg 1921 aggtactgca catggtgagg aatgccaaac tagttggcca gtctattatt gcttatctcc 1981 agaagaaggg ctatcctgaa gtggcactgc attttgtcaa ggatgagaaa actcgcttta 2041 gtctggcact ggagtgtgga aacattgaga ttgctctgga agcagccaaa gcactggatg 2101 acaagaactg ctgggaaaag ctgggagaag tggccctgct gcaggggaac caccagattg 2161 tggaaatgtg ctatcagcgt accaaaaact ttgacaaact ttccttcctg tatcttatca 2221 ctggcaactt agaaaaactt cgcaagatga tgaagattgc tgagatcaga aaggacatga 2281 gtggccacta tcagaatgcc ctatacctgg gtgatgtgtc agagcgtgtg cggatcctga 2341 agaactgtgg acagaagtcc ctggcctatc tcacagctgc tacccatggc ttagatgaag 2401 aagctgagag cctaaaggag acatttgacc cagagaagga gacaatccca gacattgacc 2461 ctaatgccaa gctgctccag ccacctgcac ctatcatgcc attggatacc aattggcctt 2521 tattgactgt atccaaagga ttttttgaag gcaccattgc cagcaaaggg aagggaggag 2581 cactggctgc tgacattgac attgacactg ttggtacaga gggctgggga gaggatgcag 2641 agctgcagtt ggatgaagat gggtttgtgg aggctacaga aggtttgggg gatgatgctc 2701 ttggcaaggg acaggaagaa ggaggtggct gggatgtaga agaagatctg gagctccctc 2761 ctgagctgga tatatcccct ggggcagctg gtggggctga agatggtttc tttgtgcccc 2821 caaccaaggg aacaagtcca actcagatct ggtgtaataa ctctcagctt ccagttgatc 2881 acatcctggc aggctctttc gaaacagcca tgcggctcct tcatgaccaa gtaggggtaa 2941 tccagtttgg cccctacaag caactgttcc tacagacata cgcccgaggc cgcacaacct 3001 atcaggctct gccctgccta ccctccatgt atggctatcc taatcgcaac tggaaggatg 3061 cagggctgaa gaatggtgta ccagctgtgg gcctgaagct taatgacctc atccaacggt 3121 tgcagctgtg ctaccagctc accacagttg gcaaatttga ggaggctgtg gaaaaattcc 3181 gttccatcct tctcagtgtg ccacttcttg ttgtggacaa taaacaagag atTGCAGAGG 3241 CCCAGCAGCT CATCACCATT TGCCGTGAGT ACATTGTGGG TTTGTCCGTG GAGACAGAAA 3301 GGAAGAAGCT GCCCAAAGAG ACTCTAGAAC AGCAGAAGCG CATCTGTGAG ATGGCAGCCT 3361 ATTTCACCCA CTCAAACCTG CAGCCTGTGC ACATGATCCT GGTGCTGCGT ACAGCCCTCA 3421 ATCTGTTCTT CAAGCTCAAG AACTTCAAGA CAGCTGCCAC CTTTGCTCGG CGCCTACTAG 3481 AACTCGGGCC CAAGCCTGAG GTGGCCCAAC AGACCCGAAA AATCCTGTCT GCCTGTGAGA 3541 AGAATCCCAC AGATGCCTAC CAGCTCAATT ATGACATGCA CAACCCCTTT GACATTTGTG 3601 CTGCATCATA TCGGCCCATC TACCGTGGAA AGCCAGTAGA AAAGTGTCCA CTCAGTGGGG 3661 CCTGCTATTC CCCTGAGTTC AAAGGTCAAA TCTGCAGGGT CACCACAGTG ACAGAGATTG 3721 GCAAAGATGT GATTGGTTTA AGGATCAGTC CTCTGCAGTT TCGCTACCAC TTTCTTGTAC 3781 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 3841 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 3901 AAGGACGACA CACTCTCAAG GACTCGCCTT GGACGCGTTA AGTCgacaat caacctctgg 3961 attacaaaat ttgtgaaaga tt