Construct: ORF TRCN0000479352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008637.1_s317c1
- Derived from:
- ccsbBroadEn_10458
- DNA Barcode:
- GCTACTAAGTCCAAACTACTGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDH12 (51294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51294 | PCDH12 | protocadherin 12 | NM_016580.3 | 99.5% | 99.5% | (many diffs) |
| 2 | human | 51294 | PCDH12 | protocadherin 12 | XM_024446106.1 | 99.5% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3627
- ORF length:
- 3561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gcaacttctg caacttctgc tggggctttt ggggccaggt ggctacttat 121 ttcttttagg ggattgtcag gaggtgacca ctctcacggt gaaataccaa gtgtcagagg 181 aagtgccatc tggtacagtg atcgggaagc tgtcccagga actgggccgg gaggagaggc 241 ggaggcaagc tggggccgcc ttccaggtgt tgcagctgcc tcaggcgctc cccattcagg 301 tggactctga ggaaggcttg ctcagcacag gcaggcggct ggatcgagag cagctatgcc 361 gacagtggga tccctgcctg gtttcctttg atgtgcttgc cacaggggat ttggctctga 421 tccatgtgga gatccaagtg ctggacatca atgaccacca gccacggttt cccaaaggcg 481 agcaggagct ggaaatctct gagagcgcct ctctgcgaac ccggatcccc ctggacagag 541 ctcttgaccc agacacaggc cctaacaccc tgcacaccta cactctgtct cccagtgagc 601 actttgcctt ggatgtcatt gtgggccctg atgagaccaa acatgcagaa ctcatagtgg 661 tgaaggagct ggacagggaa atccattcat tttttgatct ggtgttaact gcctatgaca 721 atgggaaccc ccccaagtca ggtaccagct tggtcaaggt caacgtcttg gactccaatg 781 acaatagccc tgcgtttgct gagagttcac tggcactgga aatccaagaa gatgctgcac 841 ctggtacgct tctcataaaa ctgaccgcca cagaccctga ccaaggcccc aatggggagg 901 tggagttctt cctcagtaag cacatgcctc cagaggtgct ggacaccttc agtattgatg 961 ccaagacagg ccaggtcatt ctgcgtcgac ctctagacta tgaaaagaac cctgcctacg 1021 aggtggatgt tcaggcaagg gacctgggtc ccaatcctat cccagcccat tgcaaagttc 1081 tcatcaaggt tctggatgtc aatgacaaca tcccaagcat ccacgtcaca tgggcctccc 1141 agccatcact ggtgtcagaa gctcttccca aggacagttt tattgctctt gtcatggcag 1201 atgacttgga ttcaggaaac aatggtttgg tccactgctg gctgagccaa gagctgggcc 1261 acttcaggct gaaaagaact aatggcaaca catacatgtt gctaaccaat gccacactgg 1321 acagagagca gtggcccaaa tataccctca ctctgttagc ccaagaccaa ggactccagc 1381 ccttatcagc caagaaacag ctcagcattc agatcagtga catcaacgac aatgcacctg 1441 tgtttgagaa aagcaggtat gaagtctcca cgcgggaaaa caacttaccc tctcttcacc 1501 tcattaccat caaggctcat gatgcagact tgggcattaa tggaaaagtc tcataccgca 1561 tccaggactc cccagttgct cacttagtag ctattgactc caacacagga gaggtcactg 1621 ctcagaggtc actgaactat gaagagatgg ccggctttga gttccaggtg atcgcagagg 1681 acagcgggca acccatgctt gcatccagtg tctctgtgtg ggtcagcctc ttggatgcca 1741 atgataatgc cccagaggtg gtccagcctg tgctcagcga tggaaaagcc agcctctccg 1801 tgcttgtgaa tgcctccaca ggccacctgc tggtgcccat cgagactccc aatggcttgg 1861 gcccagcggg cactgacaca cctccactgg ccactcacag ctcccggcca ttccttttga 1921 caaccattgt ggcaagagat gcagactcgg gggcaaatgg agagcccctc tacagcatcc 1981 gcagtggaaa tgaagcccac ctcttcatcc tcaaccctca tacggggcag ctgttcgtca 2041 atgtcaccaa tgccagcagc ctcattggga gtgagtggga gctggagata gtagtagagg 2101 accagggaag ccccccctta cagacccgag ccctgttgag ggtcatgttt gtcaccagtg 2161 tggaccacct gagggactca gcccgcaagc ctggggcttt gagcatgtcg atgctgacgg 2221 tgatctgcct ggctgtactg ctgggcatct tcgggttgat cctggctttg ttcatgtcca 2281 tctgccggac agaaaagaag gacaacaggg cctacaactg tcgggaggcc gagtccgcct 2341 accgccagca gcccaagagg ccccagaaac acattcagaa ggcagacatc cacctcgtgc 2401 ctgtgctcag gggtcaggca ggtgagcctt gtgaagtcgg gcagtcccac aaagatgtgg 2461 acaaggaggc gatgatggaa gcaggctggg acccctgcct gcaggccccc ttccacctca 2521 ccccgaccct gtacaggacg ctgcgtaatc aaggcaacca gggagcaccg gcggagagcc 2581 gagaggtgct gcaagacacg gtcaacctcc ttttcaacca tcccaggcag aggaatgcct 2641 cccgggagaa cctgaacctt cccgagcccc agcctgccac aggccagcca cgttccaggc 2701 ctctgaaggt tgcaggcagc cccacaggga ggctggctgg agaccagggc agtgaggaag 2761 ccccacagag gccaccagcc tcctctgcaa ccctgagacg gcagcgacat ctcaatggca 2821 aagtgtcccc tgagaaagaa tcagggcccc gtcagatcct gcggagcctg gtccggctgt 2881 ctgtggctgc cttcgccgag cggaaccccg tggaggagct cactgtggat tctcctcctg 2941 ttcagcaaat ctcccagctg ctgtccttgc tgcatcaggg ccaattccag cccaaaccaa 3001 accaccgagg aaataagtac ttggccaagc caggaggcag caggagtgca atcccagaca 3061 cagatggccc aagtgcaagg gctggaggcc agacagaccc agaacaggag gaagggcctt 3121 tggatccTGA AGAGGACCTC TCTGTGAAGC AACTGCTAGA AGAAGAGCTG TCAAGTCTGC 3181 TGGACCCCAG CACAGGTCTG GCCCTGGACC GGCTGAGCGC CCCTGACCCG GCCTGGATGG 3241 CGAGACTCTC TTTGCCCCTC ACCACCAACT ACCGTGACAA TGTGATCTCC CCGGATGCTG 3301 CAGCCACGGA GGAGCCGAGG ACCTTCCAGA CGTTCGGCAA GGCAGAGGCA CCAGAGCTGA 3361 GCCCAACAGG CACGAGGCTG GCCAGCACCT TTGTCTCGGA GATGAGCTCA CTGCTGGAGA 3421 TGCTGCTGGA ACAGCGCTCC AGCATGCCCG TGGAGGCCGC CTCCGAGGCG CTGCGGCGGC 3481 TCTCGGTCTG CGGGAGGACC CTCAGTTTAG ACTTGGCCAC CAGTGCAGCC TCAGGCATGA 3541 AAGTGCAAGG GGACCCAGGT GGAAAGACGG GGACTGAGGG CAAGAGCAGA GGCAGCAGCA 3601 GCAGCAGCAG CAGCAGCAGG TGCCTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 3661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCTACTAA 3781 GTCCAAACTA CTGTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3841 aagatt