Construct: ORF TRCN0000479390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012816.1_s317c1
- Derived from:
- ccsbBroadEn_09639
- DNA Barcode:
- CGTGATCTTCTTTGACCATACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUNDC1 (146923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146923 | RUNDC1 | RUN domain containing 1 | NM_173079.5 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 146923 | RUNDC1 | RUN domain containing 1 | XM_005257080.4 | 99.6% | 99.3% | (many diffs) |
| 3 | human | 146923 | RUNDC1 | RUN domain containing 1 | NM_001321381.3 | 99.4% | 99.1% | (many diffs) |
| 4 | human | 146923 | RUNDC1 | RUN domain containing 1 | XM_005257078.4 | 99.2% | 99% | (many diffs) |
| 5 | mouse | 217201 | Rundc1 | RUN domain containing 1 | NM_172566.4 | 86.5% | 88.7% | (many diffs) |
| 6 | mouse | 217201 | Rundc1 | RUN domain containing 1 | XM_006533035.3 | 86.2% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1905
- ORF length:
- 1839
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctttcgaa gcggctgcag agccggtaac ggtggtggcg gctgttgggc 121 caaaggcgaa agacgaagag gaggaggaag aggagccgct gccaccgtgc gaggcggtgc 181 gctgggcccc agttggggcg gtggcggagg cccggcctgg ggcaaccgcg tttttagaag 241 aggcgacggc cgaggagcct ggcgcggccc cgggctcccc gccggattcg ccgggccgga 301 cgctgcggcg gctgcgggca gagcggcggc ggctggactc ggcgctgctg gcgctgtcct 361 cgcacttcgc gcaggtgcag ttccgcctgc gccaggtggt gcgcggggcg ccggcggagc 421 agcagcgcct tctgcgggag ctcgaagact tcgccttccg cggctgccct cacgtcctag 481 gttacgaagg gcccggcgac cccgccagcg atgagggcga tgggctgcca ggggaccggc 541 cacggttgcg gggcgaggac cagagtgagc aggaaaagca agagcgtctg gaaacccaaa 601 gggagaagca gaaagaactg atactgcagc tcaagaccca gctagatgac ctggaaacgt 661 ttgcctatca agagggcagt tatgactcgc tgccacagtc cgtggtgttg gaaagacagc 721 gggtgatcat agatgagtta ataaagaaac tggacatgaa tctgaatgag gacatcagtt 781 ccctgtccac tgaagagctt cgtcagcgtg tagatgcagc agtggctcag atcgtcaacc 841 cagcccgagt caaagaacag ttggttgagc aactgaaaac tcagatccga gaccttgaga 901 tgtttatcaa cttcatccaa gatgaagtgg gaagcccctt gcagacaggt ggtggacact 961 gtgagtgcaa ggccggtggg aagacaggaa atggctgcag cagaacaggc agcagcagaa 1021 cgcctccagg aaacagcaaa acaaaggcag aggatgtgaa gaaagtccgg gagacggggc 1081 tgcacctgat gcggcgagcg ctggccgtgc tccagatctt tgctgttagc cagtttggtt 1141 gtgccacagg ccagatccct ccaaccctgt ggcagagggt ccaggctgac agagactact 1201 ctcccttgct gaagaggctg gaggtgtcag tggacagagt gaagcagcta gccatgaggc 1261 agcagccaca tgaccatgtc atcacctctg ccaacctcca ggacctctct ctgggaggca 1321 aggatgagct gactatggct gtgcggaagg agctaacggt ggctgtgagg gacctgctgg 1381 cccatggact gtatgcctcc tccccaggga tgagccttgt tatggctcct attgcttgtt 1441 tgctgccagc cttctcctcg gccccagagg ccatgcaccc gtgggagctc tttgtaaagt 1501 actaccatgc taagaacggc cgtgcttatg tggaatcccc agcccggaag ctctcccagt 1561 ccttcgccct tcctgttacg ggaggcactg ttgtcacccc caaacagagc ctactgacag 1621 ccatCCACAT GGTGCTGACA GAGCATGACC CTTTTAAGCG CAGTGCAGAC TCAGAATTGA 1681 AGGCCTTGGT GTGCATGGCA CTGAATGAGC AGCGTCTGGT GTCCTGGGTG AACCTCATCT 1741 GCAAGTCCGG GTCACTCATC GAGCCTCACT ACCAGCCCTG GAGCTACATG GCACACACAG 1801 GCTTTGAGAG TGCCCTCAAC CTGCTCAGTC GCCTCAGCAG CCTCAAGTTT AGCCTCCCTG 1861 TAGATCTGGC TGTGCGCCAG CTCAAAAACA TCAAAGATGC CTTTTGCCCA ACCTTCTTGT 1921 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1981 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2041 GAAAGGACGA CGTGATCTTC TTTGACCATA CCTAACGCGT TAAGTCgaca atcaacctct 2101 ggattacaaa atttgtgaaa gatt