Construct: ORF TRCN0000479391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005287.1_s317c1
- Derived from:
- ccsbBroadEn_10499
- DNA Barcode:
- GCCAATCGATACGCGGTAGTTGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTENP1 (11191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_000314.8 | 90.1% | 89% | (many diffs) |
| 2 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_001304717.5 | 63% | 62.3% | (many diffs) |
| 3 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_001304718.2 | 55.1% | 55% | (many diffs) |
| 4 | human | 11191 | PTENP1 | phosphatase and tensin homo... | NR_023917.1 | 28.1% | 1_871del;1524A>G;1979_3932del | |
| 5 | mouse | 19211 | Pten | phosphatase and tensin homolog | NM_008960.2 | 87% | 88.8% | (many diffs) |
| 6 | mouse | 19211 | Pten | phosphatase and tensin homolog | XM_006526769.2 | 69.2% | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1173
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg atttcctgca gaaagacttg aaggcgtata caggaacaat attgatgatg 121 tagtaaggtt tttggattca aagcataaaa accattacaa gatacacaat ctttgtgctg 181 aaagacatta tgacaccgcc aaatctaatt acagagttgc gcaatatcct tttgaagacc 241 ataacccacc acagctagaa cttatcaaac ccttttgtga agatcttgac caatggctaa 301 gtgaagatga caatcatgtt gcagcaattc actgtaaagc tggaaaggga cgaactggta 361 taatgattta tgcatattta ttacatcggg gcaaattttt aaaggcacaa gaggccctag 421 atttctatgg ggaagtaagg accagagaca aaaagggagt aactattccc agtcagaggc 481 gctatgtgta ttactatagc tacctggtaa agaatcatgt ggattataga ccagtggcac 541 tgttgtttca caagatgatg tttgaaacta ttccaatgtt cagtggcgga acttgcaatc 601 ctcagtttgt ggtctgccag ctaaaggtga agatgtattc ctccaattca ggacccacac 661 gatgggagga caagttcatg tattttgagt tccctcagcc gttacctgtg tgtggtggta 721 tcaaagtaga gttcttccac aaacagaaca agatgctaaa aaaggacaaa atgtttcact 781 tttgggtaaa tacattcttc ataccaggac cagaggaaac cTCAGAAAAA GTAGAAAATG 841 GAAGTCTATG TGATCAAGAA ATTGATAGCA TTTGCAGTAT AGAGCGTGCA GATAATGACA 901 AGGAGTATCT AGTACTTACT TTAACAAAAA ATGATCTTGA CAAAGCAAAT AAAGACAAAG 961 CCAACCGATA CTTTTCTCCA AATTTTAAGG TGAAGCTGTA CTTCACAAAA ACAGTAGAGG 1021 AGCCGTCAAA TCCAGAGGCT AGCAGTTCAA CTTCTGTAAC ACCAGATGTT AGTGACAATG 1081 AACCTGATCA TTATAGATAT TCTGACACCA CTGACTCTGA TCCAGAGAAT GAACCTTTTG 1141 ATGAAGATCA GCATACACAA ATTACAAAAG TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1321 CAATCGATAC GCGGTAGTTG CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt