Construct: ORF TRCN0000479456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004271.1_s317c1
- Derived from:
- ccsbBroadEn_13747
- DNA Barcode:
- ACGGGCATAAGCATTGTGAAACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOSMO (730094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479456
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 730094 | MOSMO | modulator of smoothened | NM_001164579.2 | 50.7% | 43.7% | (many diffs) |
| 2 | mouse | 233812 | BC030336 | cDNA sequence BC030336 | XM_006507689.2 | 56.7% | 51% | (many diffs) |
| 3 | mouse | 233812 | BC030336 | cDNA sequence BC030336 | NM_001164580.1 | 49% | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 363
- ORF length:
- 273
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGTTACTT CTGCCAGATT AATCTGGGAA 121 GAAACAGAGC ACTCACTGTG GGCCTCGTGC GACAGTGTCA AACAATCCAT GGACGAGACC 181 GGACGTGCAT CCCTCCCCGG CTTCCCCCGG AGTGGGTCAC CACACTGTTT TTTATCATCA 241 TGGGAATCAT TTCATTGACT GTCACATGTG GTTTGCTGGT GGCTTCCCAC TGGCGAAGAG 301 AAGCTACAAA ATATGCTCGA TGGATAGCAT TCACTGGAAA GTGTGTGCAT TCTTCTCATA 361 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GGGCATAAGC ATTGTGAAAC CGACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt