Construct: ORF TRCN0000479464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010829.1_s317c1
- Derived from:
- ccsbBroadEn_10603
- DNA Barcode:
- CTTATTGCATCGGGTGTCTCTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LINC00663 (284440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284440 | LINC00663 | long intergenic non-protein... | NR_026956.1 | 14.5% | 1_285del;610C>A;634_2385del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tcacccagag gcttcaaggc ccttctcacc atggtaccca ccTCCTCGAA 121 GCTGCCTCCC AAAGCCCTCT AGCTGCTGCT GCAGCCACAA CAAGTGCCAC CACCAGGGGC 181 CTTCCAGCTG CCAGTGCAGG CTTTCCAGAG CCGGGGACTG GGGAACCTGC AGTCATCACC 241 TCAGCAATTC TGGTGGCAGC CAGGCCAGCC AGGGCAGCGA TGATGACCGT AATGAAAAGT 301 GGGATGACAG CTCCACACTG GTGGATGAGT TGAGAACACC AGGCAGAGGC ATGTACCTGG 361 TCTTTGATGG TTCAGTGGAC CTGCACTACA ATTGCAGTGC AAAGTGCAAG AGTTGCCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAC TTATTGCATC GGGTGTCTCT GGGACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att