Construct: ORF TRCN0000479479

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014266.1_s317c1
Derived from:
ccsbBroadEn_07989
DNA Barcode:
TTTCTGAAATAATCGATTCCCTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCAF4 (26094)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479479
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_181340.3 99.9% 100% 1137G>A
2 human 285429 DCAF4L1 DDB1 and CUL4 associated fa... NM_001029955.4 85% 76.7% (many diffs)
3 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021214.1 83.4% 83.5% 1_234del;1371G>A
4 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_001163509.2 83.2% 83.3% 1_234del;740_742delCAG;1374G>A
5 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021212.1 83.2% 83.3% 1_234del;740_742delCAG;1374G>A
6 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021213.2 83.2% 83.3% 1_234del;740_742delCAG;1374G>A
7 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_181341.3 82% 67.3% (many diffs)
8 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_015604.4 79.7% 79.7% 1_300del;1437G>A
9 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021207.1 79.7% 79.7% 1_300del;1437G>A
10 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021208.1 79.7% 79.7% 1_300del;1437G>A
11 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_001352449.2 79.5% 79.6% 1_300del;806_808delCAG;1440G>A
12 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021201.1 79.5% 79.6% 1_300del;806_808delCAG;1440G>A
13 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021202.1 79.5% 79.6% 1_300del;806_808delCAG;1440G>A
14 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021203.1 79.5% 79.6% 1_300del;806_808delCAG;1440G>A
15 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021205.1 79.5% 79.6% 1_300del;806_808delCAG;1440G>A
16 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021206.1 79.5% 79.6% 1_300del;806_808delCAG;1440G>A
17 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_001163508.2 78.1% 78.2% (many diffs)
18 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021215.2 70.8% 70.8% 0_1ins345;792G>A
19 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_001352447.2 70.6% 70.7% 0_1ins345;161_163delCAG;795G>A
20 human 26094 DCAF4 DDB1 and CUL4 associated fa... NM_001352448.2 70.5% 66.4% (many diffs)
21 human 26094 DCAF4 DDB1 and CUL4 associated fa... XM_017021209.1 70.3% 66.2% (many diffs)
22 human 26094 DCAF4 DDB1 and CUL4 associated fa... NR_147990.2 50.4% (many diffs)
23 mouse 73828 Dcaf4 DDB1 and CUL4 associated fa... XM_006516283.2 70% 70.6% (many diffs)
24 mouse 73828 Dcaf4 DDB1 and CUL4 associated fa... NM_030246.2 65.2% 64.3% (many diffs)
25 mouse 73828 Dcaf4 DDB1 and CUL4 associated fa... XM_006516282.1 63.7% 62.9% (many diffs)
26 mouse 73828 Dcaf4 DDB1 and CUL4 associated fa... NM_001165256.1 63.6% 62.7% (many diffs)
27 mouse 73828 Dcaf4 DDB1 and CUL4 associated fa... XM_006516284.2 58.3% 58.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1251
ORF length:
1185
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gagcaagaga ctgcggctgc tccaggaaga agacagacgg aaaaagattg
121ccaggatggg atttaatgca tcttccatgc tacgaaaaag ccagctgggt tttctcaacg
181tcaccaatta ctgccattta gcccacgagc tgcgtctcag ctgcatggag aggaaaaagg
241tccagattcg aagcatggat ccctccgcct tggcaagcga ccgatttaac ctcatactgg
301cagataccaa cagtgaccgg ctcttcacag tgaacgatgt taaagttgga ggctccaagt
361atggtatcat caacctgcaa agtctgaaga cccctacgct caaggtgttc atgcacgaaa
421acctctactt caccaaccgg aaggtgaatt cggtgtgctg ggcctcgctg aatcacttgg
481attcccacat tctgctatgc ctcatgggac tcgcagagac tccaggctgt gccaccctgc
541tcccagcatc actgttcgtc aatagtcacc caggaataga ccggcctggc atgctctgca
601gtttccggat ccctggtgcc tggtcctgtg cctggtccct gaatatccaa gcaaataact
661gcttcagtac aggcttgtct cggcgggtcc tgttgaccaa cgtggtgacg ggacaccggc
721agtcctttgg gaccaacagt gatgtcttgg cccagcagtt tgctctcatg gctcctctgc
781tgtttaatgg ctgccgctct ggggaaatct ttgccattga tctgcgttgt ggaaatcaag
841gcaagggatg gaaggccacc cgcctgtttc atgattcagc agtgacctct gtgcggatcc
901TCCAAGATGA GCAATACCTG ATGGCTTCAG ACATGGCTGG AAAGATCAAG CTGTGGGACC
961TGAGGACCAC GAAGTGCGTA AGGCAGTACG AAGGCCACGT GAATGAGTAC GCCTACCTGC
1021CCCTGCATGT GCACGAGGAA GAAGGAATCC TGGTGGCAGT GGGCCAGGAC TGCTACACGA
1081GAATCTGGAG CCTCCACGAT GCCCGCCTAC TGAGAACCAT ACCCTCCCCG TACCCTGCCT
1141CCAAGGCCGA CATTCCCAGT GTGGCCTTCT CGTCGCGGCT GGGGGGCTCC CGGGGCGCGC
1201CAGGGCTGCT CATGGCTGTC GGGCAGGACC TTTACTGTTA CTCCTACAGC TACCCAACTT
1261TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1321CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1381CTTGTGGAAA GGACGATTTC TGAAATAATC GATTCCCTGC ACGCGTTAAG TCgacaatca
1441acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)