Construct: ORF TRCN0000479527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016010.1_s317c1
- Derived from:
- ccsbBroadEn_12915
- DNA Barcode:
- AGAAGGCACGCTTCAGCCGCGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFHX2 (85446)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | XM_017021715.2 | 15.5% | 14.1% | (many diffs) |
| 2 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | NM_033400.3 | 15.4% | 14% | (many diffs) |
| 3 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | XM_011537245.3 | 15.4% | 14% | (many diffs) |
| 4 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | XM_011537246.3 | 15.4% | 14% | (many diffs) |
| 5 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | XM_011537247.2 | 15.4% | 14% | (many diffs) |
| 6 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | XM_017021714.1 | 15.4% | 14% | (many diffs) |
| 7 | human | 85446 | ZFHX2 | zinc finger homeobox 2 | XM_017021716.2 | 15.3% | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1293
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaccctt aactcagcct ctaccactgg taccaccccc tcccctgggc 121 acaatgcccc gtccctgcct tcggacacct tctcctccag caccccctct gatcctgtca 181 ccaaagatcc ccctgctgcc tcctccacct ctgagaacat gaggtcctca gagccagggg 241 gacagctcct ggagtcgggc tgtggcctcg tcccaccaaa ggagattggg gagccccagg 301 aagggcctga ctgtggtcac ttcccaccaa atgacccagg ggtggaaaag gacaaggagc 361 aggaggagga agaagaaggg ctccctccca tggacctaag caaccactta ttcttcacag 421 ctggaggtga ggcctaccta gtggccaagc tgtccctgcc aggtggcagt gaactcctgt 481 taccaaaggg cttcccctgg ggtgaggcgg gcatcaagga agagcccagt ctgcccttcc 541 ttgcctaccc acccccctca cacctcactg cccttcacat ccaacatggc tttgacccaa 601 tccaaggctt tagctcttct gaccaaattc tgtcccatga tacctcagca ccatctccgg 661 ctgcctgtga ggaaaggcat ggagctttct ggagctacca gctggctcca aatccacccg 721 gagatcccaa agatggcccc atggggaaca gcgggggcaa ccacgtggcg gtcttctggc 781 tctgccttct gtgccgcctg ggtttcagca agccccaggc ctttatggat cacacacagt 841 ctcatggggt gaagctaacc cctgcccaat atcagggcct gtcaggtagc ccagctgtac 901 tccaggaggg agatgaaggc tgcaaggccc tcataagctt tctGGAGCCA AAACTCCCTG 961 CTCGCCCCTC TTCTGACATA CCCCTTGACA ACAGCAGCAC AGTGAACATG GAGGCGAATG 1021 TGGCCCAGAC AGAGGATGGC CCCCCTGAGG CAGAAGTCCA GGCCCTTATC CTCCTGGATG 1081 AAGAAGTTAT GGCCCTCAGC CCACCCTCTC CACCCACAGC CACCTGGGGC TATACCACTG 1141 CCTGTTGTGT GCGTGGGAGA CACCCTCCCG CTTGGCTGTG CTGCAACACC TGCGCACACC 1201 TGCCCACCGC GATGCCCAGG CCCAGAGGCG TCTGCAGCTG CTACAGAATG GCCCAACCAC 1261 TGAGGAAGGA CTCGCAGCTC TTCAGAGCAT CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 1441 AAGGCACGCT TCAGCCGCGC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt