Construct: ORF TRCN0000479546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000808.1_s317c1
- Derived from:
- ccsbBroadEn_04046
- DNA Barcode:
- CGCAAACATTCTATCAGCGGACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF426 (79088)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479546
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79088 | ZNF426 | zinc finger protein 426 | NM_024106.3 | 100% | 100% | |
| 2 | human | 79088 | ZNF426 | zinc finger protein 426 | XM_024451702.1 | 100% | 100% | |
| 3 | human | 79088 | ZNF426 | zinc finger protein 426 | NM_001300883.3 | 93.1% | 92.9% | 0_1ins112;2_3insCA |
| 4 | human | 79088 | ZNF426 | zinc finger protein 426 | XM_017027293.2 | 93.1% | 92.9% | 0_1ins112;2_3insCA |
| 5 | human | 79088 | ZNF426 | zinc finger protein 426 | NM_001318055.2 | 79.9% | 79.9% | 0_1ins333 |
| 6 | human | 79088 | ZNF426 | zinc finger protein 426 | XM_017027294.1 | 56.3% | 56.3% | 0_1ins726 |
| 7 | human | 79088 | ZNF426 | zinc finger protein 426 | XM_024451703.1 | 56.3% | 56.3% | 0_1ins726 |
| 8 | human | 79088 | ZNF426 | zinc finger protein 426 | XM_024451704.1 | 56.3% | 56.3% | 0_1ins726 |
| 9 | human | 79088 | ZNF426 | zinc finger protein 426 | NM_001318056.2 | 21.2% | 20.3% | 324_325ins83;354_355ins1225 |
| 10 | human | 79088 | ZNF426 | zinc finger protein 426 | XM_024451705.1 | 21.2% | 20.3% | 324_325ins83;354_355ins1225 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1728
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agctgctgat ttgtcccatg gacattatct ttctggggac ccagtttgcc 121 ttcatgaaga aaagacacca gcaggaagaa tagtggctga ctgcctaaca gattgttatc 181 aggattcagt gacctttgac gatgtggctg tggacttcac ccaggaggag tggactttac 241 tggactcaac tcagagaagc ctctacagtg acgtgatgct ggagaactac aagaacctgg 301 ccacagtagg aggtcagatc atcaaaccca gtctaatctc ttggttggaa caagaagagt 361 caaggacagt tcagggagga gttctccaag gatgggaaat gcgacttgaa acccagtggt 421 ctatacttca gcaggacttt ttgaggggtc agacatccat tgggatacaa ttggaaggaa 481 aacacaatgg aagggaactc tgtgactgtg agcaatgtgg agaagtcttc agtgaacact 541 catgccttaa gacgcacgtg agaactcaaa gtacagggaa cactcatgac tgtaatcagt 601 atggaaaaga tttccttacc ctgtgtgaga aaacctctac tggtgagaaa ctttctgagt 661 ttaatcagag tgaaaaaatc ttcagcctga caccaaatat tgtataccag agaactagca 721 cacaagaaaa gtcatttgaa tgtagtcact gtggaaaatc cttcattaat gagtcatacc 781 ttcaggcaca tatgagaact cacaatggag aaaaactcta cgaatggagg aattatgggc 841 caggttttat tgactctaca agcctttctg tgcttataga aaccctcaat gcaaaaaagc 901 cctacaaatg taaggaatgt ggaaaaggct atagataccc agcctacctc agtattcaca 961 tgcgaaccca cactggggag aaaccatatg aatgtaagga atgtgggaaa gccttcaatt 1021 attccaactc atttcagata catggaagaa ctcacactgg agagaaaccc tatgtatgta 1081 aggaatgtgg gaaagccttc actcagtact cgggccttag tatgcatgta cgatctcaca 1141 gtggagacaa gccctatgaa tgtaaggaat gtgggaaatc cttccttaca tcctcacgcc 1201 ttattcaaca tataagaact cacactggag agaagccttt tgtatgtgtt gaatgtggga 1261 aagcctttgc agtttcctca aatcttagtg gacatttgag aactcacact GAAGAGAAGG 1321 CCTGTGAGTG TAAGATATGT GGGAAAGTAT TTGGGTATCC CTCATGTCTT AATAATCACA 1381 TGCGAACGCA CAGTGCCCAG AAACCATACA CCTGTAAGGA ATGTGGGAAG GCTTTTAACT 1441 ATTCCACCCA CCTTAAAATT CACATGCGAA TCCACACTGG AGAAAAACCC TATGAGTGTA 1501 AACAATGTGG AAAGGCCTTC AGTCATTCCA GTTCATTTCA AATACATGAA AGGACTCACA 1561 CTGGAGAGAA ACCCTATGAA TGCAAGGAGT GTGGGAAAGC CTTCACGTGT TCCAGTTCCT 1621 TTAGAATTCA TGAAAAAACT CACACAGAAG AGAAACCCTA TAAATGTCAG CAATGCGGGA 1681 AAGCTTACAG TCATCCCCGT TCACTTCGAA GACATGAACA AATTCACTAC CCAACTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGACGCAAAC ATTCTATCAG CGGACCAACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt