Construct: ORF TRCN0000479575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014975.1_s317c1
- Derived from:
- ccsbBroadEn_11375
- DNA Barcode:
- AACCGCAAAATTTTAGCAGATCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMF1 (9491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479575
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323409.2 | 37.8% | 36% | 107T>G;282_364del;381_783del |
| 2 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323408.2 | 37.5% | 35.7% | 107T>G;282_364del;381_789del |
| 3 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323410.2 | 37.5% | 35.7% | 107T>G;282_364del;381_789del |
| 4 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_006814.5 | 36.4% | 34.6% | 107T>G;282_364del;381_813del |
| 5 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_178578.3 | 36.4% | 34.6% | 107T>G;282_364del;381_813del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 390
- ORF length:
- 297
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGCGGG CCTGGAGGTA CTGTTCGCAT 121 CGGCAGCGCC GGCCATCACC TGCAGGCAGG ACGCGCTCGT CTGCTTCTTG CATTGGGAAG 181 TGGTGACACA CGGTTACTGC GGCTTGGGTG TCGGTGACCA GCCGGGTCCC AATGATAAGA 241 AGTCAGAACT GCTGCCAGCT GGGTGGAACA ACAATAAAGA CCTGTATGTC CTCCGGTATG 301 AGTATAAGGA TGGGTCCAGA AAGCTCCTTG TGAAAGCCAT CACCGTGGAG AGCAGCATGA 361 TCCTCAATGT GCTGGACCTA CAAGAACAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACC 541 GCAAAATTTT AGCAGATCAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 601 gtgaaagatt