Construct: ORF TRCN0000479579
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011710.1_s317c1
- Derived from:
- ccsbBroadEn_07118
- DNA Barcode:
- TAAAGGTGGATGTAGGACAGGTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- UGDH (7358)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479579
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7358 | UGDH | UDP-glucose 6-dehydrogenase | NM_003359.4 | 99.8% | .9% | 30C>T;399A>G |
| 2 | human | 7358 | UGDH | UDP-glucose 6-dehydrogenase | XM_005262667.3 | 97.3% | 1.3% | 1_39del;69C>T;438A>G |
| 3 | human | 7358 | UGDH | UDP-glucose 6-dehydrogenase | NM_001184700.2 | 86.3% | 1.1% | 30C>T;264_265ins201 |
| 4 | human | 7358 | UGDH | UDP-glucose 6-dehydrogenase | NM_001184701.2 | 80.2% | 2.5% | 0_1ins291;108A>G |
| 5 | mouse | 22235 | Ugdh | UDP-glucose dehydrogenase | NM_009466.2 | 89.7% | .7% | (many diffs) |
| 6 | mouse | 22235 | Ugdh | UDP-glucose dehydrogenase | XM_006503862.3 | 89.7% | .7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 58
- ORF end:
- 160
- ORF length:
- 102
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaatg 61 ttggcatgtt tgaaattaag aagatctgtt gcattggtgc aggctatgtt ggaggaccca 121 catgtagtgt cattgctcat atgtgtcctg aaatcagggt aacggttgtt gatgtcaatg 181 aatcaagaat caatgcgtgg aattctccta cacttcctat ttatgagcca ggactaaaag 241 aagtggtaga atcctgtcga ggaaaaaatc tttttttttc taccaatatt gatgatgcca 301 tcaaagaagc tgatcttgta tttatttctg tgaatactcc aacaaaaacc tatggaatgg 361 ggaaaggccg ggcagcagat ctgaagtata ttgaagcttg tgctagacgc attgtgcaaa 421 actcaaatgg gtacaaaatt gtgactgaga aaagcacagt tccggtgcgg gcagcagaaa 481 gtatccgtcg catatttgat gcaaacacaa aacccaactt gaatttacag gtgctgtcca 541 accctgagtt tctggcagag ggaacagcca tcaaggacct aaagaaccca gacagagtac 601 tgattggagg ggatgaaact ccagagggcc agagagctgt gcaggccctg tgtgctgtat 661 atgagcactg ggttcccaga gaaaagatcc tcaccactaa tacttggtct tcagagcttt 721 ccaaactggc agcaaatgct tttcttgccc agagaataag cagcattaac tccataagtg 781 ctctgtgtga agcaacagga gctgatgtag aagaggtagc aacagcgatt ggaatggacc 841 agagaattgg aaacaagttt ctaaaagcca gtgttgggtt tggtgggagc tgtttccaaa 901 aggatgttct gaatttggtt tatctctgtg aggctctgaa tttgccagaa gtagctcgtt 961 attggcagca ggtcatagac atgaatgact accagaggag gaggtttgct tcccggatca 1021 tagatagtct gtttaataca gtaactgata agaagatagc tattttggga tttgcattca 1081 aaaaggacac tggtgataca agagaatctt ctagtatata tattagcaaa tatttgatgg 1141 atgaaggtgc acatctacat atatatgatc caaaagtacc tagggaacaa atagttgtgg 1201 atctttcTCA TCCAGGTGTT TCAGAGGATG ACCAAGTGTC CCGGCTCGTG ACCATTTCCA 1261 AGGATCCATA TGAAGCATGT GATGGTGCCC ATGCTGTTGT TATTTGCACT GAGTGGGACA 1321 TGTTTAAGGA ATTGGATTAT GAACGCATTC ATAAAAAAAT GCTAAAGCCA GCCTTTATCT 1381 TCGATGGACG GCGTGTCCTG GATGGGCTCC ACAATGAACT ACAAACCATT GGCTTCCAGA 1441 TTGAAACAAT TGGCAAAAAG GTGTCTTCAA AGAGAATTCC ATATGCTCCT TCTGGTGAAA 1501 TTCCGAAGTT TAGTCTTCAA GATCCACCTA ACAAGAAACC TAAAGTGTAC CCAACTTTCT 1561 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1621 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1681 GTGGAAAGGA CGATAAAGGT GGATGTAGGA CAGGTCAACG CGTTAAGTCg acaatcaacc 1741 tctggattac aaaatttgtg aaagatt