Construct: ORF TRCN0000479588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008293.1_s317c1
- Derived from:
- ccsbBroadEn_05181
- DNA Barcode:
- GAGCCCTGGCCTAATACTACATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE4A (199834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479588
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 199834 | LCE4A | late cornified envelope 4A | NM_178356.3 | 100% | 100% | |
| 2 | human | 254910 | LCE5A | late cornified envelope 5A | NM_178438.5 | 70.9% | 63.6% | (many diffs) |
| 3 | human | 353133 | LCE1C | late cornified envelope 1C | NM_178351.4 | 65.1% | 60.1% | (many diffs) |
| 4 | human | 353132 | LCE1B | late cornified envelope 1B | NM_178349.2 | 62.5% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 390
- ORF length:
- 297
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGTCCTG CCAGCAGAAC CAACAGCAGT 121 GCCAGCCCCC TCCCAAGTGT CCTATCCCCA AGTATCCCCC AAAATGTCCC TCAAAGTGTG 181 CATCCTCATG CCCACCTCCA ATCTCTTCCT GCTGTGGCTC CAGCTCTGGG GGCTGTGGTT 241 GCTGCAGCTC TGAGGGAGGT GGCTGCTGCC TGAGCCACCA CAGACACCAT AGGTCCCACT 301 GCCACAGACC CAAGAGCTCC AATTGCTATG GCAGTGGCAG TGGCCAGCAG TCTGGGGGTT 361 CTGGCTGCTG CTCTGGAGGG GGCTGTTGCT TGCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGCC 541 CTGGCCTAAT ACTACATTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt