Construct: ORF TRCN0000479620

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000185.1_s317c1
Derived from:
ccsbBroadEn_10552
DNA Barcode:
GTGGATGCGCTCGTGATGAAAAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPACA6 (147650)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479620
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 147650 SPACA6 sperm acrosome associated 6 NR_024330.1 31.3% 1_187del;235C>N;821_2017del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
702
ORF length:
633
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggccctgctg gctctggcca gtgccgtccc gtctgccctg ctggcnctgg
121ctgtcttcag ggtgcccgcc tgggcctgtc tcctctgctt cacaacctac tctgagcgcc
181tccgcatctg ccagatgttt gttgggatgc ggagccccaa gcttgaagag tgtgaggagg
241ccttcacggc cgccttccag ggcctctctg acaccgaaat cagtgaggag accatccaca
301cttcatcagt gtcctggGGA AGGTGCAGAG GGAGGGCAGG AGAGGCCCAG AGGGTCAGGC
361TGAGGGACAG ACAGAGAGAA ACAGTCAGAG GAGAAAGGCT CAAAGACCAT GAGAACAACA
421GAGACTTAGG GACAGAGAGA CACAGACAGG GGAAGACAGC AGGGCAAAGA CTCAGAGAGG
481GGAGGATGGA GAGTCAGAGA GGGGAAGATG GAGACTCAGA GAGGGGGAGG ATGGAGACTC
541AGAGAGAGAG GAAGATGGAG ACTCGGAAAG ATGGAGACTC AGGAGTATGG AGAGTCAGAG
601AGGGGAGGAT GGACACTCGG GAGGATGGAG AGTCAGGAGG ATGGAGACTC ATAGAAAGGG
661GAGGATGGAG AGTCAGGAGA GGTTGGAGAC TGGAGAGGGA ATTGCCAACT TTCTTGTACA
721AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
781AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
841AGGACGAGTG GATGCGCTCG TGATGAAAAA CACGCGTTAA GTCgacaatc aacctctgga
901ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)