Construct: ORF TRCN0000479620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000185.1_s317c1
- Derived from:
- ccsbBroadEn_10552
- DNA Barcode:
- GTGGATGCGCTCGTGATGAAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPACA6 (147650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147650 | SPACA6 | sperm acrosome associated 6 | NR_024330.1 | 31.3% | 1_187del;235C>N;821_2017del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 702
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgctg gctctggcca gtgccgtccc gtctgccctg ctggcnctgg 121 ctgtcttcag ggtgcccgcc tgggcctgtc tcctctgctt cacaacctac tctgagcgcc 181 tccgcatctg ccagatgttt gttgggatgc ggagccccaa gcttgaagag tgtgaggagg 241 ccttcacggc cgccttccag ggcctctctg acaccgaaat cagtgaggag accatccaca 301 cttcatcagt gtcctggGGA AGGTGCAGAG GGAGGGCAGG AGAGGCCCAG AGGGTCAGGC 361 TGAGGGACAG ACAGAGAGAA ACAGTCAGAG GAGAAAGGCT CAAAGACCAT GAGAACAACA 421 GAGACTTAGG GACAGAGAGA CACAGACAGG GGAAGACAGC AGGGCAAAGA CTCAGAGAGG 481 GGAGGATGGA GAGTCAGAGA GGGGAAGATG GAGACTCAGA GAGGGGGAGG ATGGAGACTC 541 AGAGAGAGAG GAAGATGGAG ACTCGGAAAG ATGGAGACTC AGGAGTATGG AGAGTCAGAG 601 AGGGGAGGAT GGACACTCGG GAGGATGGAG AGTCAGGAGG ATGGAGACTC ATAGAAAGGG 661 GAGGATGGAG AGTCAGGAGA GGTTGGAGAC TGGAGAGGGA ATTGCCAACT TTCTTGTACA 721 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 781 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 841 AGGACGAGTG GATGCGCTCG TGATGAAAAA CACGCGTTAA GTCgacaatc aacctctgga 901 ttacaaaatt tgtgaaagat t